补光对‘金观音’相关酶活性的影响

Effect of supplymentary lights on enzyme activities in ‘Jinguanyin’ tea leaves

[目的]研究补光对‘金观音’茶树叶片PPO、POD和SOD活性的影响。[方法]采用3年生砂培‘金观音’茶树为试验材料,以自然光为CK,用荧光灯(白灯、蓝灯、红灯)、LED灯和钠灯进行补光处理。在补光第3、6和9天,分别测定PPO、POD和SOD活性。[结果]各光质补光处理第6天叶片PPO活性均显著高于CK,其中钠灯补光处理第6、9天的PPO活性最高,且在第6天比CK高24.6%;各光质补光处理第3、6天叶片SOD活性均低于或显著低于CK,第9天均显著高于CK;钠灯、LED灯补光处理与CK相比,对叶片POD活性影响呈现"高-低-高"的变化趋势,第6天钠灯补光处理显著低于CK。[结论]6 d的短时间钠灯补光处理不仅未破坏‘金观音’叶片SOD、POD保护酶的平衡而造成胁迫,还能显著提高PPO活性,可为生产上采用间歇补光栽培以提高PPO活性提供参考。

[Purpose] To study the effect of supplymentary lights on the activity of PPO, POD and SOD in tea leaves of ‘Jinguanyin’. [Method] The sand cultured three year′s old ‘Jinguanyin’ tea trees were used as the experimental material. The natural light was used as a control. Fluorescent light(white light, blue light, red light), LED light and sodium light were used for supplementary light treatments. The activity of PPO, POD and SOD were measured on the 3 rd, 6 th and 9 th day with supplementary light. [Result] On the 6 th day, the activity of PPO in each supplemental light treatment was significantly higher than that of CK. Among them, and the PPO activity in tea leaves supplemented with sodium lamp lights was the highest on the 6 th and 9 th day of the treatment, and it was 24.6% higher than CK on the 6 th day. On the 3 rd and 6 th day, the SOD activity of each supplementary light treatment was lower or significantly lower than CK, but on the 9 th day, the SOD activity of each supplementary light treatment was significantly higher than CK. Compared to CK, the sodium lamp and LED lamp supplemental light treatments had an effect on POD activity, showing a high-low-high trend, and the sodium lamp supplemental light treatment was significantly lower than CK on the 6 th day. [Conclusion] The 6 days of sodium lamp supplementary light treatment did not damage the protective enzyme activity of SOD and POD to cause stress, but it significantly improved the PPO activity, so as to provide a reference for the use of intermittent light supplementation to provide a higher PPO activity in fresh tea leaves.