轮状病毒感染患儿Treg/Th17平衡与NK细胞及白细胞介素-37的表达及意义

Treg/Th17 balance,NK cells and interleukin-37 in children with rotavirus infection and their significance

目的探讨轮状病毒(Rotavirus,RV)感染患儿Treg/Th17平衡、自然杀伤(NK)细胞及白细胞介素-37(IL-37)的表达及意义。方法回顾性分析2018年1-12月重庆市妇幼保健院收治的980例RV感染患儿临床资料,将其作为试验组,另选取同期于医院体检正常的儿童120名为对照组,比较两组儿童外周血Treg、Th17细胞、T淋巴细胞亚群、NK细胞亚群比例及血清细胞因子水平。结果试验组患儿外周血Treg细胞比例及Treg/Th17均低于对照组,而Th17细胞比例高于对照组(P<0.001)。试验组患儿全血中CD3^+、CD4^+比例及CD4^+/CD8^+分别为(58.94±2.75)%、(34.08±4.05)%及(1.41±0.21)均低于对照组(P<0.001)。试验组患儿全血中CD3^-CD56^negCD16^bright、CD3^-CD56^dimCD16^bright及总NK细胞比例分别为(0.61±0.21)、(0.32±0.17)及(0.93±0.31)均低于对照组(P<0.001)。试验组患儿血清白细胞介素-18(IL-18)和IL-37分别为(214.10±6.64)pg/ml和(25.43±3.10)pg/ml均高于对照组(P<0.001)。结论RV感染患儿Treg/Th17处于失衡状态,机体免疫功能差,高水平的IL-37对RV感染有一定的保护作用。

OBJECTIVE To investigate the expression and significance of the Treg/Th17 balance,nataral killer(NK)cells and interleukin-37(IL-37)in children with rotavirus(RV)infection.METHODS The clinical data of 980 children with RV infection admitted to Chongqing Health Center for Women and Child from Jan.2018 to Dec.2018 were retrospectively analyzed,the 980 children were assigned as the experiment group,and 120 children with normal physical examination in our hospital were selected as the control group.The proportion of peripheral blood Treg,Th17 cell,T lymphocyte subsets,NK cell subsets and serum cytokine levels were compared between two groups.RESULTS The proportion of Treg cells and Treg/Th17 in peripheral blood in the experiment group were significantly lower than those in the control group,While the proportion of Th17 cells were significantly higher than that in the control group(P<0.001).The proportion of CD3^+,CD4^+and CD4^+/CD8^+in the whole blood in the experiment group were(58.94±2.75)%、(34.08±4.05)%and(1.41±0.21).respectively,significantly lower than those in the control group(P<0.001).The proportions of CD3^-CD56^negCD16^bright,CD3-CD56^dimCD16^bright and total NK cells in the whole blood in the experiment group were(0.61±0.21)、(0.32±0.17)and(0.93±0.31).respectively,significantly lower than those in the control group(P<0.001).The levels of serum interleukin-18(IL-18)and IL-37 in the experiment group were(214.10±6.64)pg/ml and(25.43±3.10)pg/ml,respectively,significantly higher than those in the control group(P<0.001).CONCLUSION The Treg/Th17 in children with RV infection is in an unbalanced state,the body’s immune function is poor.High levels of IL-37 have a certain protective effect on RV infection.