高效液相色谱法测定猪血浆、全血和血小板中五羟色胺水平及其相关性分析

Determination of 5-HT in the plasma, whole blood and platelets of weaned pigs by high performance liquid chromatograph and analysis of their correlation

本试验采集43头苏淮猪的血液样本,探究利用高效液相色谱法(HPLC)测定血浆和全血五羟色胺(5-HT)的色谱条件,测定了血浆、全血和血小板中5-HT的水平,并对它们进行了相关性分析。结果:猪血浆中5-HT浓度为(45.35±2.23)μg/mL,血小板中5-HT含量为(131.31±7.86)pg/10^9,全血中5-HT浓度为(296.87±12.81)μg/mL;相关性分析显示,血小板5-HT浓度与全血5-HT浓度存在极显著正相关性(r=0.849,P<0.01),血小板5-HT浓度与血小板数量存在极显著负相关性(r=-0.549,P<0.01),但血浆5-HT浓度与全血5-HT浓度、血小板5-HT浓度无显著相关性(P>0.05)。综上,高效液相色谱法可用于测定猪血浆、全血、血小板中5-HT的水平,血小板5-HT含量与全血5-HT浓度和血小板数量之间均存在极显著相关性。

The objective of this study was to establish a method for determination of the 5-hydroxytryptamine(5-HT) level in the plasma, whole blood and platelets of weaned piglets by high performance liquid chromatograph(HPLC).A total of 43 blood samples from Suhuai weaned pigs were collected and used.The concentration of 5-HT in the plasma was(45.35±2.23)μg/mL, in the platelets(131.31±7.86)pg/10^9, and in the whole blood(296.87±12.81)μg/mL.The Pearson correlation analysis indicated that the 5-HT level in the platelet was very positively associated with that in the whole blood(r=0.849, P<0.01), but was very negatively associated with the quantity of the platelet(r=-0.549, P<0.01).There was no significant correlation between the 5-HT levels in the plasma and the whole blood and the platelet(P>0.05).To sum up, HPLC can be used to effectively detect the 5-HT levels in the plasma, the whole blood and the platelet of weaned pigs.