红景天苷对流感病毒感染小鼠血清和肺组织炎症因子及免疫指标的影响

Influence of salidroside on serum and lung tissue inflammatory factors and immunological indexes of mice infected with influenza virus

目的探讨红景天苷对流感病毒感染小鼠血清和肺组织炎症因子及免疫指标的影响。方法选取60只雄性SPF级BALB/c小鼠,经鼻腔接种甲型流感病毒株制备流感病毒感染动物模型,并随机分为模型组和红景天苷高、低剂量组。每组各20只,红景天苷高、低剂量组分别接受50、25 mg/kg的灌胃处理,每天1次,连续2周。另选取20只小鼠经鼻腔接种生理盐水0.1 ml作对照。2周后收集各组小鼠的整个肺脏称重并计算肺指数,留取左肺下叶称重计算干湿比,取右肺组织进行炎症病理评分,采用流式细胞术检测外周血T淋巴细胞亚群比例情况,采用酶联免疫吸附法检测血清和肺组织炎症因子[肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、IL-6和C-反应蛋白(CRP)]水平。结果与对照组相比,模型组小鼠感染2周后的肺指数、炎症病理评分及血清和肺组织中炎症因子IL-1β、IL-6、TNF-α、CRP水平均升高,而干湿比、CD4^+、CD4/CD8^+水平均降低,差异有统计学意义(P<0.05);与模型组相比,红景天苷高、低剂量组的肺指数、炎症病理评分及血清和肺组织中炎症因子水平均降低,而干湿比、CD4^+、CD4/CD8^+水平均升高,差异有统计学意义(P<0.05)。除肺指数外,红景天苷高剂量组的以上指标均优于低剂量组,差异有统计学意义(P<0.05)。结论红景天苷对流感病毒感染小鼠的肺部炎症有一定的改善效果并降低血清与肺组织中炎症相关因子释放,可能与其增强病毒感染引起的免疫功能降低有关。

OBJECTIVE To explore the influence of salidroside on serum and lung tissue inflammatory factors and immunological indexes of mice infected with influenza virus. METHODS A total of 60 male SPF BALB/c mice were inoculated with influenza A virus strain through nasal cavity to prepare influenza virus infection animal model and were randomly divided into the model group, the high-dose salidroside group and the low-dose salidroside group, with 20 in each group.The high-dose salidroside group and the low-dose salidroside group were respectively treated with 50 and 25 mg/kg of intragastric administration once a day for two consecutive weeks.Another 20 mice were inoculated with 0.1 ml saline through nasal cavity and were set as control.Two weeks after infection, the whole lungs of the mice in each group were weighed and the lung indexes were calculated.The dry-wet ratio was calculated by left lower lobe weighing.Inflammatory pathology was scored by right lung tissue.The percentages of peripheral blood T lymphocyte subsets were detected by flow cytometry.The levels of serum and lung tissue inflammatory factors, including tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), IL-6 and C-reactive protein(CRP) were determined by means of enzyme-linked immunosorbent assay. RESULTS As compared with the control group, the lung indexes, inflammatory pathological scores and serum and lung tissue IL-1β, IL-6, TNF-α and CRP of the model group were elevated after the mice were infected for 2 weeks, while the dry-wet ratio, CD4^+ level and CD4/CD8^+ were reduced, and there were significant differences(P<0.05).As compared with the model group, the lung indexes, inflammatory pathological scores and serum and lung tissue inflammatory factors of the high-dose salidroside group and the low-dose salidroside group were reduced, while the dry-wet ratio, CD4^+ level and CD4/CD8^+ were increased, and there were significant differences(P<0.05).All of the above indexes of the high-dose salidroside group were better than those of the low-dose salidroside group except for the lung indexes, and there were significant differences(P<0.05). CONCLUSION Salidroside can achieve certain effect on improvement of pulmonary inflammation of the mice infected with influenza virus and reduce the release of and serum and lung tissue inflammatory factors, which may be associated with the decline of immune function induced by the viral infection.