鸢尾素FNDC5在肝癌组织中的表达及与肿瘤侵袭的关系

Expression of FNDC5 in liver cancer tissues and its relationship with clinical parameters

目的探讨鸢尾素FNDC5在肝细胞肝癌(hepatocellular carcinoma,HCC)中的表达水平及其与HCC患者临床病理因素和预后的关系。方法采用免疫组化、qRT-PCR检测FNDC5在HCC中的表达情况,分析其表达水平与临床病理因素及和预后的关系。结果FNDC5主要表达于肝癌细胞的胞浆中,胞核弱表达。30例肝癌组织中,FNDC5阴性0例,FNDC5弱阳性5例,FNDC5阳性19例,FNDC5强阳性6例。qRT-PCR法检测显示,在发生脉管侵袭的肝癌组织中FNDC5高表达。在FNDC5高表达的HCC中脉管侵袭的发生率为30%(9/30),显著高于FNDC5低表达组的6.7%(2/30),差异有统计学意义(χ2=15.026,P<0.05)。肝癌组织中FNDC5水平的高低与年龄、性别、乙肝病毒感染(HBsAg)、甲胎蛋白(AFP)水平等无明显相关性(P>0.05)。结论肝癌组织中FNDC5含量的高低与肝癌是否发生脉管侵袭密切相关,可预测术后肝癌复发。

Objective To investigate the expression of FNDC5 in hepatocellular carcinoma(HCC)and its relationship with clinicopathological characteristics and prognosis of HCC patients.Methods Immunohistochemistry and qRT-PCR were used to detect the expression of FNDC5 in HCC tissues,and the relationship between its expression and clinicopathological parameters and prognosis.Results FNDC5 was mainly expressed in the cytoplasm of HCC cells,weakly expressed in the nucleus.Among the 30 liver cancer tissues,FNDC5 was weakly positive in 5,positive in 19,and strongly positive in 6 cases.QRT-PCR assay showed that FNDC5 was highly expressed in HCC tissues with vascular invasion.The incidence of vascular invasion in the high-expression of FNDC5 was 30%(9/30),which was significantly higher than that of the FNDC5 low-expression group[6.7%(2/30),]and the difference was significant(χ2=15.026,P<0.05).There was no significant correlation between FNDC5 level and age,sex,HBsAg,and alpha-fetoprotein(AFP)level in HCC(P>0.05).Conclusion FNDC5 level in the liver cancer tissues is closely related to the occurrence of vascular invasion.