高脂饮食对大鼠免疫功能影响及热灭活益生菌改善作用的初探

Preliminary Studies on Effect of High-fat Diet on Rat Immunity and Possible Improvement by Heat Inactivated Probiotics

初步探究高脂饮食对机体免疫功能的影响,以及热灭活副干酪乳杆菌N1115(N1115菌)的改善作用。24只雄性SD大鼠随机分为对照组、高脂组和N1115组(n=8/组),分别饲喂普通、高脂饲料及高脂饲料和热灭活N1115菌。每周计算日平均膳食、能量摄入量和空腹体重。第0、5、10和18周测量大鼠空腹血糖(FBG)、甘油三酯(TG)和总胆固醇(TC)。第18周采集血清,检测部分细胞因子。处死后取肝、脾、肺、肾周和睾周脂肪称重。相较于对照组,高脂组和N1115组日均摄食量显著降低(P<0.05),日均能量摄入显著增高(P<0.05),体重差异无统计学意义(P>0.05),高脂组的脂体比显著增高(P<0.05)。第5、10和18周时,高脂组TC降低程度均小于对照组(P<0.05),高脂组整体TG显著升高(P<0.05),第18周N1115组TC和TG与对照组无统计学差异。高脂组血清脂联素、IL-1β、IL-6和TNF-α均显著降低(P<0.05),N1115组各指标趋近于对照组。热灭活副干酪乳杆菌N1115对长期高脂饮食导致的脂代谢及免疫功能异常有改善作用。

This study was conducted to explore if high-fat diet could affect the immunity of host animal and the heat-killed Lactobacillus paracasei N1115(N11115) could improve them. 24 male SD rats were randomly divided into control group, high-fat group and N1115 group(n=8/group). They were fed with normal, high-fat diets and high fat diets with heat killed N1115, respectively. Daily average diet, energy intake, and fasting weight were calculated weekly. Fasting blood glucose(FBG), triglyceride(TG) and total cholesterol(TC) were measured at weeks 0, 5, 10 and 18. Serum was collected at week 18 and serum cytokines were detected. After the sacrificed, liver, spleen, lung, perirenal and testicular fat were weighed. Compared with the control group, the daily averages of food intake was significantly lower in the highfat group and N1115 group(P<0.05), while the daily average of energy intake was significantly increased(P<0.05). There was no significant difference in body weight(P>0.05). However, the lipid ratio was significantly increased by high-fat(P<0.05). At the 5 th, 10 th and 18 th week, TC was reduced less in the high-fat groups than that in the control group(P<0.05), and the overall TG in the high-fat group was significantly increased(P<0.05). There was no statistical difference at the 18 th week in TC and TG between N1115 group and the control group. Serum adiponectin, IL-1β, IL-6 and TNF-α were significantly decreased in the high fat group(P<0.05), while those in N1115 group was close to the control group. Heat-killed Lactobacillus paracasei N1115 can improve lipid metabolism and immune dysfunction caused by long-term high-fat diet.