发酵乳杆菌CECT5716计数方法比较

Comparison of CECT5716 Count Methods of Lactobacillus Fermentans

针对中外乳酸菌菌落数检测标准的差异,从固体菌粉的溶解情况以及培养基成分入手,通过比对菌落生长情况找出问题所在并提出可行性改进意见。结果表明,两种标准中所用混匀方式对菌落计数无影响;冷冻保藏的固体菌粉在后续实验操作前37℃下复溶45 min能够提高其活力,是造成中外差异的因素之一;复溶时用1%缓冲蛋白胨水进行溶解对缩短中外标准菌落数检测结果有积极作用;在培养基中加入适量营养物质能够显著提升菌落生长数量。

In view of the differences in the testing standards of lactobacillus colony number in China and foreign countries, starting from the dissolution of solid bacterial powder and the composition of medium, the problems were found by comparing the growth of bacterial colonies and the feasible improvement. The results showed that the mixing method used in the two standards had no effect on the colony count.The resolubilization of solid bacterial powder after freezing can improve its vitality. The dissolution of 1% buffer peptone water can improve the detection results of colony number. Adding appropriate nutrients to the medium can significantly increase the colony.