人源性宫颈癌、子宫内膜癌、卵巢癌异种移植动物模型的建立

Establishment of xenotransplantation animal models of human-derived cervical cancer, endometrial cancer and ovarian cancer xenograft

目的构建人来源的宫颈癌(cervical cancer,CC)、子宫内膜癌(endometrial cancer,EC)、卵巢癌(ovarian cancer,OC)人源性肿瘤异种移植动物模型,为研究和开发新药及个体化治疗提供实验模型。方法收集2018年2月至2019年4月新疆医科大学第一附属医院CC、EC、OC患者各5例的新鲜手术切除标本,移植至重度免疫缺陷(immunodeficiency,NOG)小鼠和非肥胖糖尿病/重症联合免疫缺陷(non-obese diabetes/severe combined immunodeficiency,NOD/SCLD)小鼠皮下,监测荷瘤小鼠体重和肿瘤体积,对长至500~1 000 mm^3大小的肿瘤进行传代移植,通过苏木精-伊红染色法(hematoxylin-eosin staining,HE)染色及免疫组化(immunohistochemistry,IHC)验证移植肿瘤组织与患者肿瘤组织的病理学一致性。结果本研究收集并移植15例CC、EC、OC肿瘤标本,成功构建CC、EC、OC PDX模型8例,建模成功率为53%。结论模型较好地保留了原发肿瘤的特征,为后续研究开发CC、EC、OC新的治疗方案、临床药物筛选以及个体化治疗提供了实验平台。

Objective Human-derived cervical cancer(cervical cancer, CC), endometrial cancer(EC), and ovarian cancer(OC) PDX animal models were constructed to provide experimental models for research and development of new drugs and individualized treatments.Methods Freshly excised specimens from patients with clinical CC, EC, and OC were taken and transplanted into mice with severe immunodeficiency(NOG) and non-obese diabetes/severe combined immunodeficiency(NOD/SCID) mice.Subcutaneously, monitored body weight and tumor volume of tumor-bearing mice, and perform subculture transplantation for tumors up to(500~1 000) mm^3 in size, the hematoxylin-eosin staining(HE) staining and immunohistochemistry(IHC) were used to verify the pathological consistency between the transplanted tumor tissue and the patient’s tumor tissue.Results In this study, 15 CC, EC, and OC tumor specimens were collected and transplanted, and 8 CC, EC, and OC PDX models were successfully constructed with a success rate of 53 %.Conclusion The model retains the characteristics of the primary tumor well, and provides an experimental platform for subsequent research and development of new treatment schemes for CC, EC, and OC, clinical drug screening, and personalized treatment.