人参皂苷Rh2通过Egr-1/TRL4/mTOR信号通路抑制食管癌细胞Eca-109增殖、迁移和EMT

Ginsenoside Rh2 inhibits proliferation,migration and EMT of esophageal cancer cell Eca-109 through Egr-1/TRL4/mTOR signaling pathway

目的:研究人参皂苷Rh2对食管癌细胞Eca-109增殖、迁移和上皮间质转化(epithelial-mesenchymal transition,EMT)的作用以及作用机制。方法:CCK-8法检测人参皂苷Rh2对食管癌细胞Eca-109增殖的影响;细胞划痕实验检测人参皂苷Rh2对食管癌Eca-109细胞迁移的影响;Western blot检测EMT相关蛋白E-cadherin、Vimentin和Slug的蛋白表达水平。结果:人参皂苷Rh2能够显著抑制Eca-109细胞的增殖,且呈剂量依赖性;此外,人参皂苷Rh2显著抑制E-cadherin、Vimentin和Slug的蛋白表达,并抑制Eca-109细胞迁移;人参皂苷Rh2显著抑制Egr-1、TRL4和mTOR的蛋白表达;进一步的研究结果表明人参皂苷Rh2通过抑制Egr-1/TRL4/mTOR信号通路抑制食管癌细胞Eca-109增殖、迁移和EMT。结论:人参皂苷Rh2能够抑制食管癌细胞Eca-109的增殖、迁移和EMT,其作用机制是通过介导Egr-1/TRL4/mTOR信号通路来实现的。这一结果能够为治疗食管癌的进一步研究提供分子基础。

Objective:To study the effects of ginsenoside Rh2 on proliferation,migration and EMT of esophageal cancer cell Eca-109 and its mechanism.Methods:CCK-8 method was used to detect the effects of ginsenoside Rh2 on the proliferation of Eca-109 cells.Cell scratches assay was used to detect the mobility of Eca-109 cells.Western blot was used to detect the expression levels of E-cadherin,Vimentin and Slug proteins related to EMT.Results:The results showed that ginsenoside Rh2 could significantly inhibit the proliferation of Eca-109 cells in a dose-dependent manner.In addition,ginsenoside Rh2 significantly inhibited the protein expression of E-cadherin,Vimentin and Slug,and inhibited the migration of Eca-109 cells.Ginsenoside Rh2 significantly decreased the protein expression of Egr-1,TRL4 and mTOR.The results showed that ginsenoside Rh2 inhibited the proliferation,migration and EMT of Eca-109 cells via inhibiting Egr-1/TRL4/mTOR signaling pathway.Conclusion:Ginsenoside Rh2 inhibits the proliferation,migration and EMT of esophageal cancer cell Eca-109 by mediating Egr-1/TRL4/mTOR signaling pathway.This result provides a molecular basis for further research on the treatment of esophageal cancer.