lnc SNHG20和miR-495在胶质瘤中的表达关系及临床意义

The expressions and clinical significances of lnc SNHG20 and miR-495 in human glioma

目的:探讨lnc SNHG20和miR-495在人胶质瘤中的表达关系及临床意义。方法:通过实时荧光定量PCR(qRT-PCR)检测85例人胶质瘤组织和85例正常脑组织中lnc SNHG20和miR-495的表达水平,并分析lnc SNHG20和miR-495表达与临床病理特征的关系,采用Spearman相关分析法分析人胶质瘤组织中lnc SNHG20和miR-495表达相关性,Kaplan-Meier法评估lnc SNHG20和miR-495与患者生存期的关系,采用Cox回归模型分析影响患者预后因素。结果:与正常脑组织相比,人胶质瘤组织中lnc SNHG20表达水平显著升高(P<0.05),miR-495表达水平显著下降(P<0.05)。lnc SNHG20和miR-495表达与胶质瘤患者年龄、性别以及肿瘤位置无关(P>0.05),与肿瘤直径和肿瘤分级相关(P<0.05)。Spearman相关分析结果显示,lnc SNHG20和miR-495表达呈负相关(r=-0.795,P<0.05)。Kaplan-Meier生存分析结果显示,lnc SNHG20高表达患者3年总存活率明显低于lnc SNHG20低表达患者(P<0.05),miR-495高表达患者3年总存活率明显高于miR-495低表达患者(P<0.05)。lnc SNHG20和miR-495是影响胶质瘤患者预后的独立因素。结论:人胶质瘤组织中lnc SNHG20和miR-495表达异常,两者呈负相关,且与胶质瘤的恶性程度、患者的生存期及预后关系密切,可能成为胶质瘤诊断、治疗及预后的新靶点。

Objective:To investigate the expressions and clinical significances of lnc SNHG20 and miR-495 in human glioma.Methods:The expression levels of lnc SNHG20 and miR-495 in 85 human glioma tissues and 85 normal brain tissues were detected by real-time fluorescence quantitative PCR(qRT-PCR).The relationships between lnc SNHG20 and miR-495 expressions with clinicopathological features were analyzed.Spearman correlation analysis was used to analyze the correlation between lnc SNHG20 and miR-495 expression in human glioma tissues.Kaplan-Meier method was used to assess the relationships between lnc SNHG20 and miR-495 with the survival of patients.Cox regression model was used to analyze the prognostic factors.Results:Compared with normal brain tissues,the expression of lnc SNHG20 was significantly higher in human glioma tissues(P<0.05),and the expression of miR-495 was significantly lower(P<0.05).The expressions of lnc SNHG20 and miR-495 were not correlated with age,gender and tumor location(P>0.05),but correlated with tumor diameter and tumor grade(P<0.05).Spearman correlation analysis showed that the expressions of lnc SNHG20 and miR-495 were negatively correlated(r=-0.795,P<0.05).Kaplan-Meier survival analysis showed that the 3-year overall survival rate of patients with lnc SNHG20 high expression was significantly lower than that of patients with lnc SNHG20 low expression(P<0.05),and the 3-year overall survival rate of patients with miR-495 high expression was significantly higher than that of patients with miR-495 low expression(P<0.05).lnc SNHG20 and miR-495 were independent prognostic factors in glioma patients.Conclusion:The expressions of lnc SNHG20 and miR-495 in human glioma tissues are abnormal,and the two are negatively correlated,and closely related to the malignancy,survival time and prognosis of glioma.They may become new targets for diagnosis,treatment and prognosis of glioma.