摘要
目的探讨micro RNA-145-3p(miR-145-3p)在先天性巨结肠(HD)中的表达及其发挥作用的可能分子机制。方法选取2013年2月-2018年6月南方医科大学珠江医院40例HD病变段肠管(HD病变肠管组)及40例正常肠管(正常肠管组)组织标本。采用qRT-PCR检测组织标本miR-145-3p相对表达量。过表达细胞miR-145-3p后,CCK-8法检测细胞的增殖情况;Transwell检测细胞迁移情况;双荧光素酶报告基因实验、Western blotting及免疫组织化学染色明确miR-145-3p对靶基因GDNF的影响。结果HD病变肠管组miR-145-3p相对表达量高于正常肠管组(P<0.05)。过表达细胞miR-145-3p后,细胞增殖能力下降(P<0.05),细胞迁移能力降低(P<0.05);靶基因预测软件筛选出GDNF是miR-145-3p的潜在靶点;双荧光素酶报告基因实验证实,miR-145-3p可以与GDNF的3’-UTR结合(P<0.05);Western blotting检测证实,miR-145-3p可使SH-SY5Y细胞的GDNF蛋白相对表达量降低(P<0.05);而免疫组织化学染色证实,GDNF在miR-145-3p高表达的HD组织标本中存在高频低表达。结论miR-145-3p可以抑制神经母细胞的增殖及迁移,是一个重要的HD分子标志物,其作用机制可能与抑制GDNF蛋白表达有关。
Objective To investigate the relationship between miR-145-3p and Hirschsprung disease(HD),and to explore the possible molecular mechanism.Methods In this study,a total of 80 human colon tissues including 40 HD stenotic colon segments and 40 matched normal colon segments who undertook operative treatment were collected from Zhujiang Hospital of Southern medical University,and qRT-PCR was used to detect the relative expression of miR-145-3p in colon tissues.Cell Counting Kit-8(CCK-8)assay and Transwell assay were employed to investigate the biological function of miR-145-3p in human SH-SY5Y cell lines.Bioinformatic analysis,dualluciferase reporter assay,Western blot and immunohistochemistry were performed to evaluate the target for miR-145-3p.Results We found that ganglion cell numbers were reduced while miR-145-3p was upregulated in HD tissues compared to that in normal colon tissues(P<0.05).MiR-145-3p overexpression inhibited cell proliferation(P<0.05)and migration(P<0.05).Bioinformatic software data revealed that miR-145-3p could directly target the 3′-UTR of GNDF.Dual-luciferase reporter assay confirmed that the 3′-UTR of GDNF was a direct target to miR-145-3p(P<0.05).Moreover,an increased level of miR-145-3p was inversely correlated with decreased levels of GDNF protein in cells(P<0.05)and tissues.Conclusions Our study demonstrates that aberrant expression of miR-145-3p might play a crucial role in the development of HD partly by regulating GDNF expression.
作者
吴凯
陈钦明
王健俊
何继贤
余岱岳
路羿
杨六成
Kai Wu;Qin-ming Chen;Jian-jun Wang;Ji-xian He;Dai-yue Yu;Yi Lu;Liu-cheng Yang(Department of Pediatric Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou,Guangdong 510282,China)
出处
《中国现代医学杂志》
CAS
2020年第6期18-23,共6页
China Journal of Modern Medicine
基金
广东省自然科学基金(No.2017A030310113)
广东省科技发展专项资金项目(No.2017A020215172)
广东省医学科研基金(No.A2016014)
南方医科大学青年科技人员培育项目(No.PY2016016)。