食源性相关腹泻患者粪副溶血性弧菌检测结果分析

Detection and results analysis of fecal Vibrio parahaemolyticus in patients with foodborne diarrhea

目的了解多重荧光PCR检测食源性相关腹泻患者粪副溶血性弧菌与增菌培养分离法检测结果的关系以及毒力基因携带情况。方法比较多重荧光PCR与培养分离法对200例食源性相关腹泻患者粪副溶血性弧菌的检测,在培养鉴定中使用VITEK 2 Compact全自动微生物分析系统(法国bio Mérieux公司)的GN配套鉴定卡及AST-GN09药敏卡,并进行血清学分型。结果增菌培养分离法的检出率为36.00%(72/200),所有菌株均能被多重荧光PCR鉴定出tlh基因,其中毒力基因携带tdh+/trh+占2.78%(2/72)、tdh+/trh-占88.89%(64/72)、tdh-/trh+占5.55%(4/72);tdh-/trh-占2.78%(2/72)。血清型O3∶K6最多,占58.33%(42/72)、其次O4∶K8占8.33%(6/72)。结论多重荧光PCR比较适合于食源性相关腹泻患者粪副溶血性弧菌的快速鉴定与毒力检测,也应重视培养分离法对鉴定与药敏的地位,努力优化选择性培养基及培养条件,加强主动监测。

Objective To explore the relationship between detecting fecal Vibrio parahaemolyticus by multiplex fluorescent PCR and the results of enrichment culture isolation in patients with foodborne diarrhea and to study the toxin gene.Methods Fecal Vibrio parahaemolyticus in 200 patients with foodborne diarrhea were detected by multiplex fluorescent PCR and an enrichment culture isolation method.VITEK-2 compact automatic microbial analysis system(France bio Mérieux company)and the fastidious bacteria identification card were matching in the cultivation and identification,and serological typing were done.Results The detection ratio of culture isolation method was 36.00%(72/200)and all these strains could be identified by multiplex fluorescent PCR tlh gene.tdh+/trh+accounted for 2.78%(2/72),tdh+/trh-accounted for 88.89%(64/72),tdh-/trh+accounted for 5.55%(4/72),and tdh+/trh+accounted for 2.78%(2/72).Serotype O3∶K6 was the largest,accounting for 58.33%(42/72),followed by O4:K8(8.33%)(8/72).Conclusion Multiplex fluorescent PCR is more suitable for rapid identification and toxin gene detection of fecal Vibrio parahaemolyticus in foodborne diarrhea patients.We should also attach importance to the cultivation of separation method for identification and drug sensitivity and try hard to optimize selective medium and culture conditions to strengthen active monitoring.