真菌性角膜炎的实验室常规检测方法比较

Laboratory Routine Test Method for Fungal Keratitis

目的比较真菌性角膜炎采用墨汁-KOH湿片法和荧光染色检测的结果。方法选取2017年10月至2018年10月运城市眼科医院收治的68例真菌性角膜炎患者作为研究对象,采用随机数字表法分为两组,各34例。对照组采用墨汁-KOH湿片法镜检方法检测,观察组采用荧光染色方法检测,对比检测结果。结果观察组治疗前阴性率2.9%、阳性率97.1%。对照组治疗前阴性率29.4%、阳性率70.6%。两组比较差异有统计学意义(P<0.05)。观察组治疗后阴性率97.1%、阳性率2.9%,对照组治疗后阴性率94.1%、阳性率5.9%,两组比较差异无统计学意义(P>0.05)。结论采用荧光染色法对真菌性角膜炎进行检测,可有效提高疾病检出率。

Objective To compare the results of detection of fungal keratitis by wet ink-KOH method and fluorescence stainingd.Methods A total of 68 patients with fungal keratitis admitted to Yuncheng ophthalmic hospital from October 2017 to October 2018 were selected as the study objects.They were divided into two groups by random number table method,34 cases in each group.The control group was detected by ink KOH wet film method and the observation group was detected by fluorescence staining method.Results In the observation group,the negative rate was 2.9%and the positive rate was 97.1%.In the control group,the negative rate was 29.4%and the positive rate was 70.6%.There was significant difference between the two groups(P<0.05).The negative rate was 97.1%and the positive rate was 2.9%in the observation group and 94.1%in the control group.The positive rate was 5.9%.There was no significant difference between the two groups(P>0.05).Conclusion The detection of fungal keratitis by fluorescence staining can effectively improve the detection rate of fungal keratitis.