血清HSP70、NTN1、CRABP2联合检测诊断早期原发性支气管肺癌的研究

Combined detection of serum HSP70,NTN1 and CRABP2 in the diagnosis of early primary bronchogenic carcinoma

目的探讨血清中热休克蛋白家族70(HSP70)、神经轴突导向因子(NTN1)、细胞维甲酸结合蛋白2(CRABP2)联合检测对早期原发性支气管肺癌的诊断价值。方法纳入2017年1月至2019年1月该院收治的90例早期原发性支气管肺癌患者为研究组、90例体检健康者为对照组。检测2组人员血清中HSP70、NTN1、CRABP2的表达量。用受试者工作特征曲线(ROC曲线)的曲线下面积(AUC)分析血清中HSP70、NTN1、CRABP2检测在早期原发性支气管肺癌中的诊断意义。结果早期原发性支气管肺癌患者血清中HSP70、NTN1、CRABP2的表达量均高于体检健康者,差异有统计学意义(P<0.05)。早期原发性支气管肺癌患者血清中HSP70的表达量与肺部感染存在相关性(P<0.05);血清中NTN1和CRABP2的表达量与累及胸膜或心包膜存在相关性(P<0.05)。血清中HSP70、NTN1、CRABP2单向诊断早期原发性支气管肺癌患者和体检健康者的灵敏度为88.00%、93.00%、87.00%,特异度分别为90.00%、93.00%、88.00%。血清中HSP70、NTN1、CRABP2联合检测对早期原发性支气管肺癌的诊断的灵敏度为88.89%,特异度为90.00%,准确度为89.44%。结论血清中HSP70、NTN1、CRABP2的表达量检测可作为早期原发性支气管肺癌的潜在标志。

Objective To investigate the value of serum heat shock protein family 70(HSP70),netrin 1(NTN1)and cellular retinoic acid binding protein 2(CRABP2)in the diagnosis of early primary bronchogenic carcinoma.Methods Ninety patients with early primary bronchogenic carcinoma admitted to our hospital from January to September 2019 were enrolled in the study group and 90 healthy subjects were included in the control group.The expression levels of HSP70,NTN1 and CRABP2 in the serum of the two groups were detected.The AUC of the receiver operating characteristic curve(ROC curve)was used to analyze the diagnostic significance of HSP70,NTN1 and CRABP2 in early primary bronchogenic carcinoma.Results The expression levels of HSP70,NTN1 and CRABP2 in serum of patients with early primary bronchogenic carcinoma were higher than those of healthy subjects,the difference was statistically significant(P<0.05).The expression of HSP70 in serum of patients with early primary bronchogenic carcinoma was correlated with pulmonary infection(P<0.05).The expression of NTN1 and CRABP2 in serum was correlated with pleural or pericardium(P<0.05).The sensitivity of HSP70,NTN1,and CRABP2 in serum for early diagnosis of primary bronchogenic lung cancer patients and healthy subjects was 88.00%,93.00%,and 87.00%,and the specificities were 90.00%,93.00%,and 88.00%,respectively.The sensitivity of serum HSP70,NTN1 and CRABP2 in the diagnosis of early primary bronchogenic carcinoma was 88.89%,specificity was 90.00%,and accuracy was 89.44%.Conclusion The detection of HSP70,NTN1 and CRABP2 in serum can be used as a potential marker for early primary bronchogenic carcinoma.