干扰SET8在胃癌细胞肿瘤进展和顺铂敏感性中的作用

The role of interfering SET8 expression in tumor progression and sensit ivity of gastriccancer cells to cisplatin

目的探究SET8对胃癌的进展以及对顺铂敏感性的影响。方法采用免疫组织化学和蛋白质免疫印迹分析胃癌癌组织中SET8表达,采用qRT-PCR检测胃癌细胞中SET8 mRNA水平;胃癌BGC-823细胞转染SET8-shRNA后,采用CCK-8法检测细胞增殖,采用细胞集落形成实验检测细胞集落形成能力,采用transwell法检测细胞的迁移和侵袭能力;最后,采用CCK-8法检测干扰SET8表达对胃癌BGC-823细胞顺铂敏感性的影响。结果胃癌组织及胃癌细胞中SET8表达明显升高;干扰SET8表达可抑制胃癌BGC-823细胞增殖、集落形成、迁移与侵袭,并提高胃癌细胞对顺铂敏感性。结论干扰SET8表达可抑制胃癌细胞进展并提高顺铂敏感性。

Objective To investigate the role of SET8 in the progression and cisplatin sensitivity of gastric cancer.Methods The protein level of SET8 in gastric cancer tissues was analyzed by immunohistochemistry and western blot.The mRNA level of SET8 in gastric cancer cells was analyzed by qRT-PCR.After transfected with SET8-shRNA,the cell proliferation of gastric cancer BGC-823 cells was measured by CCK-8 assay;the abilities of colony forming,migration and invasion were evaluated by colony formation assay and transwell assay,respectively.At last,CCK-8 assay was used to detect the effect of interfering with SET8 expression on cisplatin sensitivity of gastric cancer BGC-823 cells.Results The expression of SET8 was significantly increased in gastric cancer tissues and gastric cancer cells.Interfering with SET8 inhibited proliferation,colony formation,migration and invasion of gastric cancer BGC-823 cells as well as increased the sensitivity of gastric cancer cells to cisplatin.Conclusion Interference with SET8 can inhibit the tumor progression and increase the sensit ivity of gastric cancer cells to cisplatin.