骨肽对骨质疏松大鼠的保护作用

Protective Effect of Bone Peptide in Osteoporosis Rats

研究骨肽对维甲酸所致大鼠骨质疏松的保护作用.采用70 mg/kg维甲酸灌胃给予SD大鼠持续2 w构建骨质疏松模型,分别灌胃给与大鼠40 mg/kg依替膦酸二钠溶液,1.25,2.50和5.00 g/kg骨肽溶液4 w后,取大鼠股骨进行HE染色、骨密度检测、干重与灰重及骨钙磷质量分数测定,取血清和尿液进行生化指标检测.结果显示,模型组大鼠骨密度、骨小梁密度降低,灌胃给予中、高剂量骨肽后显著升高.与模型组比较,3个剂量的骨肽均能显著升高股骨干重与灰重、骨钙磷质量分数;能升高大鼠血清中的骨碱性磷酸酶(BALP)与骨钙素(BGP)的质量分数,降低血清中抗酒石酸酸性磷酸酶5b(TRACP-5b)水平.模型组大鼠尿液中的羟脯氨酸(HOP)质量分数明显升高,灌胃给予低剂量骨肽后显著降低.阳性药在各指标中均表现出明显的治疗效果,不同剂量的骨肽显示出了一定程度的剂量依赖性保护作用,2.50 g/kg骨肽溶液对维甲酸所致大鼠骨质疏松的治疗效果最明显.

To study the protective effect of bone peptide on osteoporosis induced by retinoic acid in rats,an osteoporosis model was established by orally administration to SD rats with 70 mg/kg retinoic acid for two weeks.After 4-week oral administration of 40 mg/kg etedronate disodium or three doses of bone peptide(1.25,2.50 and 5.00 g/kg)respectively,the rat femurs were collected for determination of HE,bone density,dry and ash weight,contents of Ca and P.Serum and urine were collected for assay of biochemical indicators.Results show that,compared with the control group,the bone density and bone trabeculae density are decreased in the model group,and the index is significantly increased after oral administration of medium and high doses of bone peptide.Three doses of bone peptide can significantly increase dry and ash weight of femur,contents of Ca and P.They also can increase the contents of BALP and BGP in rat serum,and decrease the level of TRACP-5b.The content of HOP in urine of rats in model group was increased dramatically and decreased significantly after oral administration of low dose of bone peptide.The positive drug showed obvious therapeutic effect in each index.Different doses of bone peptide could show a dose-dependent protective effect,while 2.50 g/kg bone peptide has the most obviously therapeutic effect on osteoporosis induced by retinoic acid in rats.