组学技术分析肉鸡胚胎发育过程中肝脏蛋白表达的变化

The Changes of Hepatic Proteins during Chicken Embryonic Development Based on Proteomics Analysis

旨在探讨肉鸡胚胎在发育中后期肝脏蛋白水平的变化。本试验选取120枚重量为(65±0.2) g的罗氏308肉鸡种蛋,分为14胚龄(E14)和出壳1日龄(H1)两组,每组3个重复,每个重复20枚种蛋。孵化至E14胚龄和H1日龄时,采集肝脏组织样本,应用相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantitation,iTRAQ)结合生物信息学分析技术筛选核心差异蛋白。结果显示,在E14和H1日龄之间共筛选出10个核心差异蛋白,主要促进脂肪酸降解(上调ACOX1、ACSL1、ACSL5、CPT1A和ECI2)和糖异生(上调ALDH3A2、FBP1、FBP2、GPI和PGM2,下调LDHB和ALDH9A1)。试验结果提示,胚胎发育的主要能量供给途径是脂肪酸降解,而不是糖酵解。此外,糖异生增多,促进糖原储存以应对出壳以及营养环境变化。

This study aimed to explore the changes of protein levels in chicken embryos during the middle and late developmental stages. One hundred and twenty fertilized eggs with(65±0.2)g were selected and randomly divided into 2 groups(E14 and H1), 3 replicates per group, and 20 eggs in each replicate. The liver tissues were collected at E14 and H1, respectively. Proteomics approach based on isobaric tags for relative and absolute quantitation(iTRAQ) was employed to screen the core differential proteins in liver. The results showed that 10 core differential proteins with different expression levels were identified in H1 compared with E14, these proteins mainly enhanced fatty acid degradation(upregulated ACOX1, ACSL1, ACSL5, CPT1 A and ECI2),and gluconeogenesis(upregulated ALDH3 A2, FBP1, FBP2, GPI and PGM2,downregulated LDHB and ALDH9 A1). These results indicate that the main energy supply pathway for embryo development is fatty acid degradation, not glycolysis. In addition, increased gluconeogenesis promotes glycogen storage to cope with shelling and changes in the nutritional environment.