桑白皮生物碱与辛伐他汀联用对高脂血症大鼠作用研究

Synergistic effects of Cortex Mori alkaloids combined with Simvastatin in rats with hyperlipidaemia

目的:探讨桑白皮生物碱与辛伐他汀联用对高脂血症金黄地鼠的降脂作用。方法:以高脂饲料喂养金黄地鼠6周建立高脂血症模型,将40只金黄地鼠随机分为正常组、模型组、桑白皮生物碱组(25 mg·kg-1·d-1),辛伐他汀组(15 mg·kg-1·d-1)和联合用药组(桑白皮生物碱25 mg·kg-1·d-1,辛伐他汀15 mg·kg-1·d-1),灌胃给药2周后心脏采血测定TG、TC、LDL-C、HDL-C等血生化指标以及ALT、AST、CRP、NO、Lp-PLA2水平;肝脏HE染色观察病理改变,油红O染色观察肝细胞脂质沉积情况。结果:与模型组相比,联合用药组的TC水平明显降低(P<0.05);辛伐他汀组和联合用药组的LDL-C水平明显降低(P<0.05);联合用药组NO水平呈明显升高(P<0.05),且与桑白皮生物碱组和辛伐他汀组相比差异均具有统计学意义;桑白皮生物碱组、辛伐他汀组以及联合用药组均能够明显降低CRP的水平(P<0.05);联合用药能够明显降低Lp-PLA2的水平(P<0.05)。肝脏HE染色和油红O染色结果显示药物治疗后均能够改善肝脏的病变情况和脂质沉积,且联合用药组的改善情况要优于单独使用二者(P<0.05)。结论:辛伐他汀联合桑白皮生物碱给药治疗对于改善高脂血症金黄地鼠的脂质水平和炎症程度更为有效,桑白皮生物碱可以作为辛伐他汀的辅助用药,有望为临床上治疗高脂血症提供新的选择。

AIM:To explore the lipid lowering effect of Cortex Mori alkaloids combined with simvastatin on hyperlipidemia rats.METHODS:Hyperlipidemia model was established by feeding golden hamsters with high-fat diet for 6 weeks,and 40 golden hamsters were randomly divided into normal group,model group,Cortex Mori alkaloids group(CMA,25 mg·kg-1·d-1),simvastatin group(15 mg·kg-1·d-1)and combination group(Cortex Mori alkaloids,25 mg·kg-1·d-1,simvastatin 15 mg·kg-1·d-1).2 weeks after the intragastric administration,cardiac blood sampling was used to determine the blood biochemical indexes such as TG,TC,LDL-C,HDL-C,as well as the levels of ALT,AST,CRP,NO,Lp-PLA2.Liver HE staining was used to observe pathological changes,and liver cell lipid deposition was observed by oil red O staining.RESULTS:Compared with the model group,TC levels in combination group were significantly reduced(P<0.05),LDL-C levels in simvastatin group and the combination group were significantly reduced(P<0.05).The level of NO in the combined group was significantly increased(P<0.05),and the difference was statistically significant compared with that in Cortex Mori alkaloids group and simvastatin group(P<0.05).The levels of CRP were significantly decreased in Cortex Mori alkaloids group,simvastatin group and the combination group(P<0.05).The level of Lp-PLA2 in combination group was significantly reduced(P<0.05).The results of liver HE staining and oil red O staining showed that both of them could improve the hepatic lesion and lipid deposition after drug treatment,and the improvement of the combination group was better than that of the two groups alone.CONCLUSION:Simvastatin combined with Cortex Mori alkaloids can effectively decrease lipid level and inflammatory level of golden hamstrel with hyperlipidemia.Cortex Mori alkaloids can be used as the auxiliary drug of simvastatin,which is expected to provide a new choice for clinical treatment of hyperlipidemia.