盐、干旱胁迫对拟南芥WRKY71基因突变体种子萌发的影响

Effects of Salt and Drought Stresses on Seed Germination of Arabidopsis WRKY71 Gene Mutant

WRKY71是影响拟南芥开花和分枝发育的重要转录因子,而其在种子萌发中的作用尚不清楚。本研究以拟南芥野生型(Col-0)、WRKY71过表达突变体(D27)和T-DNA插入突变体(wrky71)种子为材料,分析正常条件、盐胁迫、干旱胁迫处理对种子萌发的影响。结果表明:正常条件下三种材料萌发一致;盐胁迫下尤其是LiCl处理的wrky71种子萌发率优于Col-0,而D27则低于Col-0;干旱胁迫1d时,D27明显低于Col-0,随后三者的萌发率达到一致。这说明,D27种子萌发对盐和干旱胁迫较Col-0敏感,而wrky71相对不敏感。

WRKY71 is an important transcription factor which regulates the process of flowering and branch development of Arabidopsis,however,its function on seed germination is not clear.In this study,Arabidopsis wild type(Col-0),WRKY71 overexpression mutant(D27)and T-DNA insertion mutant(wrky71)were used to analyze the effects of normal conditions,salt stress and drought stress on seed germination.The results showed that the germination rates of the three materials were the same under normal condition.Under salt stress,especially under LiCl treatment,the germination rate of wrky71 was higher than that of Col-0,while that of D27 was lower compared with Col-0.The germination rate of D27 was obviously lower than that of Col-0 after one-day drought stress,then that of different materials was similar.The results indicated that the seed germination of D27 was more sensitive to salt stress and drought stress compared with Col-0,while that of wrky71 was relatively insensitive.