精子DNA碎片与复发性流产相关性的系统评价

Systematic review of the relationship between sperm DNA fragmentation and recurrent spontaneous abortion

目的系统评价精子DNA碎片与复发性流产之间的相关性。方法以"Sperm DNA integrity","The sperm DNA integrity","sperm DNA fragmentation index","sperm DNA fragmentation","SDF","DFI","Habitual Abortion","Habitual Abortions","Miscarriage,Recurrent","Recurrent Miscarriage","Recurrent Miscarriages","Abortion,Recurrent","Recurrent Abortion","Recurrent Abortions","Recurrent Early Pregnancy Loss","recurrent spontaneous abortion"为主题词及自由词检索PubMed、The Cochrane Library、Embase、Web of science,检索时间为各数据库建库至2019年7月10日,对最终纳入的非随机对照研究根据纽卡斯尔-渥太华评分(NOS)进行质量评价,运用系统评价方法阐明两者之间的相关性。结果经过仔细的搜索和排除后,最后纳入28篇非随机对照研究。结果显示:应用Acridine orange test(AO)、Comet assay(Comet)、Sperm Chromatin Structure assay(SCSA)、Sperm Chromatin Dispersion(SCD)、The terminal deoxyuridine nick labeling assay(TUNEL)方法测定精子DNA碎片,复发性流产与精子DNA碎片存在明显的相关性。结论复发性流产患者配偶精子DNA碎片指数是高于配偶无复发性流产史的正常生育男性。

Objective To explore the correlation between sperm DNA fragmentation and recurrent miscarriages.Methods Using the key words"Sperm DNA integrity","The sperm DNA integrity","sperm DNA fragmentation index","sperm DNA fragmentation","SDF","DFI","Habitual Abortion","Habitual Abortions","Miscarriage,Recurrent","Recurrent Miscarriage","Recurrent Miscarriages","Abortion,Recurrent","Recurrent Abortion","Recurrent Abortions","Recurrent Early Pregnancy Loss","recurrent spontaneous abortion",we searched the database of PubMed,The Cochrane Library,Embase,Web of science(up to July 2019)for the related studies.Using the Newcastle-Ottawa Scale,we assessed the quality of the non-randomized controlled trials.Using method of systematic reviews,we clarified the correlation between sperm DNA fragmentation and recurrent miscarriages.Results After careful search and exclusion,the twenty-eight studies were finally included.The results based on Acridine orange test(AO),Comet assay(Comet),Sperm Chromatin Structure assay(SCSA),Sperm Chromatin Dispersion(SCD)and The terminal deoxyuridine nick labeling assay(TUNEL)for analysis of sperm DNA fragments revealed a significant correlation between recurrent abortion with sperm DNA fragment.Conclusion Existing researches suggest that sperm DNA fragments were associated with recurrent spontaneous abortion.