橡胶树林长期喷施硫磺对土壤细菌多样性的影响

Effects of Long-term Sulfur Spraying on Soil Bacterial Diversity in Rubber Plantations

分析橡胶林园长期大量喷施硫磺对橡胶林园土壤细菌群落组成及多样性的影响。采用高通量测序技术测定西双版纳橡胶园研究区4个硫磺喷洒浓度梯度和1个空白对照的橡胶林土壤细菌的16S序列,测定土壤细菌群落组成及多样性的变化,并分析环境因子对细菌组成的影响。结果表明,在OTU水平上,序列分析得到的物种数(Sobs)以空白对照组土壤样本最低,其与喷施0.5、1.0倍和1.5倍硫磺土壤样本有显著差异;各组间的覆盖度指数(Coverage)、丰富度指数(Chao)和多样性指数(Simpson和Shannon-wiener)无显著差异;增加硫磺喷施浓度对Verrucomicrobia(疣微菌门)、Planctomycetes(浮霉状菌门)、Latescibacteria菌群生长有促进作用,1.5~2.0倍硫磺到达促进作用的顶点;土壤有机质与细菌群落间无显著相关性。通过高通量测序技术分析喷施不同浓度梯度硫磺的土壤,速效P、全K和pH是影响土壤细菌多样性的重要因素。当前硫磺喷施量对橡胶林土壤细菌α多样性有显著促进作用,但对橡胶林土壤细菌的组成无显著影响。

The effects of long-term and large-scale sulfur spraying on the composition and diversity of soil bacterial community in rubber plantations were analyzed.High throughput sequencing technique was used to determine the 16s sequence of soil bacteria in four sulfur spraying concentration gradients and a blank control in the rubber plantation research area of Xishuangbanna.The changes of soil bacterial community composition and diversity with sulfur spraying at different concentration gradients were determined,and the effects of environmental factors on bacterial composition were analyzed.At the OTU(operational taxonomic units)level,the number of species(Sobs)obtained by sequencing analysis was the lowest in the blank control group,which was significantly different from the soil samples sprayed with 0.5,1.0,and 1.5 times sulfur.There were no significant differences in the indices of Coverage,Chao and Simpson and Shannon-wiener among groups.Increased sulfur spraying concentration promoted the growth of Verrucomicrobia,Planctomycetes and Latescibacteria,and 1.5 to 2.0 times sulfur was used to promote the growth of Verrucomicrobia,Planctomycetes and Latescibacteria to the apex of action.There was no significant correlation between soil organic matter and bacterial community.High-throughput sequencing technology was used to analyze the soil sprayed with sulfur of different concentration gradients.Available phosphorus,total potassium and pH were important factors affecting soil bacterial diversity.Current sulfur spraying amount had a significant effect on bacterial alpha diversity in rubber tree forest soil,but had no significant effect on bacterial composition in rubber forest soil.