膳食结构与硒对大鼠糖代谢的作用

Effects of dietary structure and selenium concentration on glucose metabolism in rats

目的观察不同硒浓度和膳食结构对糖代谢的影响,探究膳食结构与硒对糖代谢的影响.方法选用6周龄Wistar大鼠150只,雌雄各半,随机分为15组,每组分别给予3种硒浓度和5种膳食结构的饲料共计15种进行喂养,每周测定1次体质量,4周后处死,测定其血清硒含量及血糖,分析不同硒浓度及膳食结构对体质量、血清硒含量及血糖的影响.结果硒浓度与膳食结构的作用存在性别差异.雌鼠体质量增长速度明显小于雄鼠,差异有统计学意义(P<0.001);雌鼠血清硒含量低于雄鼠,且除普通对照组外其他各组雌鼠与雄鼠的血清硒含量差异有统计学意义(P<0.05),不同硒浓度和膳食结构对血清硒含量均有影响,且对雌鼠血清硒含量的影响存在交互作用(P=0.003);雌鼠的血糖基本均低于雄鼠,且在高蛋白组、高糖组、高脂组雌鼠与雄鼠血糖差异均有统计学意义(P<0.05).不同硒浓度和膳食结构对血糖的影响存在交互作用(P<0.05).结论硒浓度和膳食结构对大鼠的血清硒含量和血糖的作用存在一定的交互作用,但具体作用机制有待进一步探究.

Objective To explore the effects of different selenium concentrations and dietary structures on glucose metabolism.Methods A total of 1506-week-old Wistar rats were randomly divided into 15 groups,with 5 female and 5 male rats in each group.Each group was fed for 4 weeks with 3 different concentrations of selenium and 5 different dietary structures,altogether 15 kinds of feedings.The weight of rats was measured every week.After 4 weeks,the rats were sacrificed,the serum selenium and glucose levels were detected,and the effects of different selenium concentrations and dietary structures on glucose metabolism were analyzed.Results There were differences in effects between male and female rats:female rats had smaller growth rate of body weight than male rats(P<0.001);female rats had lower selenium level than male rats,and the difference was statistically significant except in the ordinary control group(P<0.05).Different selenium concentrations and dietary structures had effects on serum selenium level,and there was an interaction effect in female rats(P=0.003).Female rats had lower serum glucose level than male rats,and the differences were statistically significant in high-protein group,high-glucose group and high-fat group(P<0.05).There was an interaction between the effects of different selenium concentrations and dietary structures on blood glucose(P<0.05).Conclusion There is an interaction between selenium concentration and dietary structure on serum selenium and glucose levels in rats,but the specific mechanism remains to be further explored.