D-半乳糖增加树鼩肠道IL-18的表达及肠道菌群失调

D-Galactose increases the expression of IL-18 in the gut of tree shrews and deregulates intestinal flora

目的分析D-半乳糖作用下,树鼩肠组织IL-18的表达和肠道菌群的变化情况。方法 10只雄性树鼩随机分为正常组和D-半乳糖组(n=5)。处理组腹腔注射D-半乳糖(0. 6 g/kg) 8周;正常组按相同操作注射生理盐水。分离回肠、盲肠和结肠并收集其内容物,免疫组化检测IL-18表达情况;16S rDNA高通量测序分析肠内容物细菌DNA序列。结果 D-半乳糖组的回肠、盲肠和结肠的IL-18表达均显著性升高(P<0. 05)。D-半乳糖组盲肠、结肠的肠杆菌科和结肠气单胞菌科丰度显著性升高;结肠双歧杆菌科丰度下降。结论 D-半乳糖增加肠道炎症因子IL-18表达和肠道菌群紊乱,推测树鼩肠道菌群失调引起IL-18表达升高,促进肠道炎症反应。

Objective To analyze the expression of IL-18 and changes of intestinal flora in tree shrews under the action of D-galactose. Methods Ten male tree shrews were randomly divided into normal and D-galactose groups( n = 5).Intraperitoneally injected D-galactose( 0. 6 g/kg) for 8 weeks was applied to the D-galactose group. Tree shrews in the normal group were injected with normal saline in the same manner. The ileum of the small intestines and the cecum and colon of the large intestines were separated,and their contents were collected. Expression of IL-18 was detected by immunohistochemistry. The bacterial 16 S r DNA sequence in intestinal contents was analyzed by high-throughput sequencing. Results Immunohistochemistry showed that the expression of IL-18 in the ileum,cecum,and colon was significantly increased in the D-galactose group( P < 0. 05). The abundances of enterobacteriaceae and aeromonas in the cecum and colon were increased significantly in the D-galactose group. The abundance of bifidobacteriaceae was decreased in the colon of the D-galactose group. Conclusions D-Galactose increases the expression of intestinal inflammatory factor IL-18 and causes intestinal flora disorder,suggesting that the imbalance of intestinal flora in tree shrews caused the increased expression of IL-18 and promoted the intestinal inflammatory response.