CX3CL1在含突变P301L转基因小鼠脑组织中的表达及意义

Expression and significance of CX3CL1 in brain tissue in mutant P301L transgenic mice

目的:探讨炎症趋化因子CX3CL1(fractalkine)在含突变p301L转基因小鼠脑组织中的表达及作用。方法:分别取9月龄含突变p301L转基因小鼠和9月龄野生型小鼠各10只。使用Western blot检测小鼠脑组织中CX3CL1蛋白的表达。取脑组织制作冰冻切片,使用抗p-tau、CX3CL1抗体行免疫荧光检测。结果:与9月龄野生型小鼠相比,9月龄转基因小鼠的脑内CX3CL1明显上调。免疫荧光共表达结果显示CX3CL1与p-tau共表达。结论:CX3CL1在含突变p301L转基因小鼠脑组织中表达上调,可能与tau导致的神经损伤的炎症机制有关。

Objective:To investigate the expression and function of CX3CL1,an inflammatory chemokine,in the brain tissue of mutant P301L transgenic mice. Methods:A total of 10 mutant P301L transgenic mice aged 9 months and 10 wild-type mice aged 9 months were selected. Western blot was used to measure the expression of CX3CL1 in brain tissue. Brain tissue samples were collected to prepare frozen sections,and immunofluorescence assay was performed with anti-p-tau and anti-CX3CL1 antibodies. Results:Compared with the wild-type mice,the transgenic mice had a significant increase in the expression of CX3CL1 in brain tissue. The immunofluorescence assay showed the co-expression of CX3CL1 and p-tau. Conclusion:CX3CL1 is significantly up-regulated in the brain tissue of mutant P301L transgenic mice and may play a role in the inflammatory mechanism of nerve injury induced by tau.