NLRP3炎症小体和氧化应激在新生儿缺氧缺血性脑病患者胎盘组织中的表达及其临床意义

Expression and clinical significances of NLRP3 inflammasomes and oxidative stress in placenta of patient with neonatal hypoxic-ischemic encephalopathy

目的研究新生儿缺氧缺血性脑病(HIE)患者胎盘组织中NLRP3炎性小体表达和氧化应激状态,探讨NLRP3炎性小体和氧化应激在HIE中的临床意义。方法选择广州市增城区人民医院2010年7月~2019年5月HIE患者胎盘组织(HIE组)及正常胎盘组织(正常组)各30例,免疫组化法检测两组NLRP3表达。取两组新鲜胎盘组织各10例,测定丙二醛(MDA)和超氧化物歧化酶(SOD)浓度。并对两组胎盘组织进行病理学评估。结果HIE组胎盘组织中有合体滋养叶细胞结节和有细胞滋养叶细胞的绒毛数量均高于正常组(P<0.05),而具有血管合体细胞膜的绒毛数少于正常组(P<0.05)。HIE组胎盘组织中的NLRP3表达量明显高于正常组,差异有统计学意义(P<0.05)。HIE组的SOD活力低于正常组(P<0.05),MDA含量高于正常组(P<0.05)。NLRP3与SOD活力呈负相关(r=-0.63,P=0.00),而与MDA含量呈正相关(r=0.74,P=0.00)。结论HIE新生儿胎盘组织中NLRP3炎症小体表达明显增加并存在异常氧化应激,两者存在相关性,其可能是新生儿HIE发生的原因。

Objective To investigate the expression and oxidative stress of NLRP3 inflammasomes in the placenta of neonates with hypoxic-ischemic encephalopathy(HIE),and to investigate the clinical significance of NLRP3 inflammasome and oxidative stress in HIE.Methods From July 2010 to May 2019,30 cases of HIE placental tissue(HIE group)and 30 cases of normal placental tissue(normal group)were selected from Zengcheng District People′s Hospital,Guangzhou,NLRP3 expression was detected by immunohistochemistry.The concentrations of malondialdehyde(MDA)and superoxide dismutase(SOD)were measured in 10 fresh placental tissues,placental tissues of the two groups were pathologically evaluated.Results The number of villi with cytosygotrophoblast nodules and cytotrophoblast cells in the placenta tissues of neonates in HIE group was higher than that of normal group(P<0.05).The number of villi with cytosomal membrane was less than that of normal group(P<0.05).The expression level of NLRP3 in HIE group neonatal placenta was significantly higher than that in normal group,and the difference was statistically significant(P<0.05).SOD activity in HIE group was lower than that in normal group(P<0.05),and MDA content was higher than normal group(P<0.05).NLRP3 was negatively correlated with SOD activity(r=-0.63,P=0.00),while positively correlated with MDA content(r=0.74,P=0.00).Conclusion There is a significant increase in the expression of NLRP3 inflammasomes in the placenta of neonates with HIE and abnormal oxidative stress,both of which are correlated,which may be the cause of neonatal HIE.