主穹隆蛋白通过抑制破骨细胞分化在牙周炎中起骨保护作用

MVP plays a protective role in periodontitis by inhibiting osteoclast differentiation and reducing bone resorption

目的:通过建立小鼠实验性牙周炎模型及体外骨髓间充质干细胞(BMMSCs)破骨细胞向诱导,探讨主穹隆蛋白(MVP)在牙周炎骨吸收中的作用。方法:MVP基因敲除(MVP-/-)和野生型(WT)C57BL/6小鼠分别局部注射脂多糖(LPS)以建立实验性牙周炎模型,通过micro CT扫描、耐酒石酸酸性磷酸酶(TRAP)染色等方法检测骨吸收程度。同时,体外分离培养MVP-/-与WT C57BL/6小鼠的BMMSCs,并诱导其向破骨细胞分化,通过TRAP染色、麦胚凝集素(WGA)染色等方法观察MVP对BMMSCs破骨向分化及骨吸收活性的影响。结果:在LPS诱导的小鼠实验性牙周炎中,MVP-/-组小鼠牙周炎骨吸收更为明显,且在注射区域内可见更多破骨细胞;体外实验证明,MVP-/-组小鼠的BMMSCs分化形成更多的破骨细胞,且骨吸收更明显。结论:MVP可以抑制破骨细胞分化,在牙周炎中起骨保护作用。

Objective:To investigate the role of major vault protein(MVP)in periodontitis by establishing an experimental model of periodontitis on mice and osteoclast induction of bone marrow mesenchymal stem cells(BMMSCs)in vitro.Methods:A model of experimental periodontitis was established by local injection of lipopolysaccharide(LPS)into wild-type mice(WT)and MVP knockout(MVP-/-)mice.The severity of periodontitis was detected by micro-CT and TRAP staining.In vitro,the role of MVP in osteoclast differentiation was verified by TRAP staining and Wheat Germ Agglutinin(WGA)staining after cultured with RANKL and M-CSF.Results:In experimental periodontitis induced by LPS,MVP-/-group developed more bone resorption and more osteoclasts could be seen in the injection area.In vitro experiments showed that knockout MVP promoted osteoclast differentiation and bone resorption.Conclusions:MVP plays a protective role in periodontitis by inhibiting osteoclast differentiation.