3D打印纳米纤维/藻酸盐基水凝胶的制备与表征

Preparation and characterization of 3D-printable nanocellulose/alginate hydrogels

以2,2,6,6-四甲基哌啶氧化物(TEMPO)氧化细菌纤维素(TOBC)作为增强体,制备了一系列的纳米纤维/藻酸盐(SA)基纳米复合水凝胶,研究了TOBC对纳米复合水凝胶的微观形貌、压缩、溶胀、生物相容性等性能的影响。通过流变实验与3D打印实验探究TOBC/SA纳米复合水凝胶的可印刷能力。结果表明,TOBC的加入显著增强了SA水凝胶的压缩性能。当TOBC含量为50%时,水凝胶压缩强度可达260.9 kPa,压缩性能最优。TOBC增强了混合油墨的非牛顿性,并使触变恢复能力最高达到83%,可3D打印制造出结构可控的复杂结构。纳米复合水凝胶表现出优异的细胞相容性,在生物医用领域特别是组织再生具有潜在的应用前景。

A series of nanocellulose/sodiumalginate(SA)nanocomposite hydrogels were prepared using 2,2,6,6-tetramethylpiperidinyl-1-oxyl(TEMPO)oxidized bacterial cellulose(TOBC)as nanoreinforcement.The effects of TOBC on the micro-morphology,compression,swelling,and biocompatibility of nanocomposite hydrogels were studied.Furthermore,the printability of TOBC/SA nanocomposite hydrogel printing ink through rheological experiments and 3D printing experiments were explored.The results showed that the addition of TOBC significantly enhanced the compression properties of the SA hydrogel.When the TOBC content was 50%,the hydrogel could reach the optimal compression performance with the compressive strength of 260.9 kPa.The presence of TOBC enhanced the non-newtonian properties of hybrid pringting inks and achieved thixotropic recovery of up to 83%,enabling to produce complex constructs with controlled structures by 3D printing.Nanocomposite hydrogels showed excellent cell compatibility and had potential applications in the biomedical field,especially in tissue regeneration.