褪黑素减轻吸烟大鼠颈动脉的氧化损伤

Melatonin attenuates the oxidative injury of smoking rat carotid arteries

目的探讨褪黑素能否减轻吸烟大鼠颈动脉的氧化损伤。方法将20只大鼠随机分为对照组、吸烟组(建立大鼠吸烟模型)和褪黑素(5 mg/kg)干预组。7 d后取颈动脉,荧光探针2',7'-DCFH-DA检测颈动脉内活性氧(ROS)含量;Western blot检测颈动脉内NF-κB、eNOS、MCP-1、VCAM-1和ICAM-1的表达。结果吸烟能明显诱导大鼠颈动脉内ROS的增加(P<0.01);褪黑素干预组ROS含量明显回降(P<0.01);吸烟组颈动脉内eNOS的表达明显低于对照组(P<0.01);褪黑素干预组颈动脉内eNOS的表达明显回升(P<0.01);吸烟组颈动脉内的NF-κB、MCP-1、VCAM-1和ICAM-1的表达明显高于对照组(P<0.01);褪黑素干预组的NF-κB、MCP-1、VCAM-1和ICAM-1的表达明显回降(P<0.05)。结论吸烟能明显诱导大鼠颈动脉内ROS的增多及氧化损伤,褪黑素能通过NF-κB通路减轻吸烟大鼠颈动脉的氧化损伤。

Objective To investigate the effect of melatonin on oxidative injury of rat carotid arteries after balloon injury.Methods Twenty male sprague-dawley rats were randomized into control,melatonin,smoking,and smoking plus melatonin.An established protocol of smoking was carried out.The carotid arteries were collected 7 days later.The average content of ROS was measured.And Western blot was used to detected the expression of NF-κB,MCP-1,VCAM-1 and ICAM-1.Results Smoking significantly induced the increase of ROS and the expression of NF-κB,MCP-1,VCAM-1 and ICAM-1 in rat carotid arteries.Moreover,cigarette smoke exposure inhibited the expression of eNOS.Melatonin minimized the effect resulted from smoking.Conclusions Melatonin may attenuate the the oxidative injury and the expression of inflammatory mediators in rat carotid arteries resulted from smoking.