紫叶丹胶囊对实验兔肝硬化模型的研究

Study of Ziyedan Capsule on Experimental Liver Cirrhosis in Rabbits

目的研究了解紫叶丹胶囊对四氯化碳致兔肝硬化的影响。方法选取实验兔45只,分为模型组、治疗组和对照组,各15例。除对照组外,其余两组动物均采用皮下注射CCl4造模。治疗组用紫叶丹胶囊灌胃,用药方法为0.78 g/kg,1次/d,灌胃给药连续8周。模型组和对照组以等体积的纯净水灌胃。HE、Masson染色检查肝组织病理变化,全自动生化分析仪检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)、总蛋白(TP)、总胆红素(TBIL)水平,ELISA法测定血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原肽(PⅢNP)、Ⅳ型胶原(CⅣ)水平。结果与对照组比较,模型组ALT、AST、TBIL、HA、LN、PⅢNP、CⅣ水平显著升,ALB、TP水平显著降低(P<0.05)。结论紫叶丹胶囊对四氯化碳致肝硬化兔具有一定的疗效,可能与改善肝脏功能和纤维化指标、促进肝组织自愈有关。

Objective To study the effect of Ziyedan Capsule on carbon tetrachloride-induced liver cirrhosis in rabbits.Methods A total of 45 experimental rabbits were divided into model group,treatment group and control group,each with 15 cases.Except for the control group,the other animals were modeled by subcutaneous injection of CCl4.In the treatment group,Ziziedan capsule was administered intragastrically at a dose of 0.78 g/kg,once a day for 8 weeks,and the model group and the normal group were administered intragastrically with the same volume of purified water.HE and Masson staining were used to examine the pathological changes of liver tissue.ALT,AST,ALB,TP and TBIL were measured by automatic biochemical analyzer.Ha,LN,PⅢNP and CⅣwere measured by ELISA.Results Compared with the control group,the levels of ALT,AST,TBIL,HA,LN,PIIINP and CIV in the model group increased significantly,and the levels of ALB and TP decreased significantly(P<0.05).Conclusion Ziyedan Capsule has certain curative effect on carbon tetrachloride-induced cirrhosis in rabbits,which may be related to improving liver function and fibrosis index and promoting liver tissue self-healing.