摘要
采用Fmoc固相合成策略,合成了胡蜂蜂毒肽(COOH-Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2)。以Wang树脂为载体,HBTU-HOBt为缩合剂,按照其氨基酸序列依次缩合,最终用切割试剂将其从树脂上切割下来,得到粗肽,经RP-HPLC纯化得到目标肽,纯度97.6%。经HR-MS(EI)分析,确定产物为胡蜂蜂毒肽。
Mastoparan(COOH-Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH2) was synthesised by Fmoc-solid phase synthesis strategy(Fmoc-SPSS). Taking Wang resin as the carrier and HBTU-HOBt as the condensation agent, the peptide was successively condensed according to its amino acid sequence. Finally, it was cleaved from the resin by cutting reagent to obtain the crude peptide, which was purified by RP-HPLC to obtain the target peptide. The target peptide was obtained and confirmed by HPLC(purity 97.6%) and HR-MS(EI).
作者
丁靖
赵昱
任成
张炼
胡婧雯
方维臻
陆群
DING Jing;ZHAO Yu;REN Cheng;ZHANG Lian;HU Jing-wen;FANG Wei-zhen;LU Qun(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;Yunnan Provincial Key Laboratory of Medical Insects and Spiders Resources for Development and Utilization,Dali University,Dali 671000,China)
出处
《合成化学》
CAS
北大核心
2020年第2期128-132,共5页
Chinese Journal of Synthetic Chemistry
基金
国家科技支撑计划课题(VQ99QT1113Y17001)
中央高校基本科研业务费科技创新项目(2682016CX105)。
关键词
胡蜂蜂毒肽
固相合成
4-甲基哌啶
氨基酸
序列
切割试剂
mastoparan
solid-phase peptide synthesis
4-pipecoline
amino acid
sequence
cleavage reagent
