不同包装、储藏处理羌活饮片的UPLC指纹图谱

UPLC Fingerprint of Notopterygh Rhizoma et Radix Pieces with Different Packaging and Storage Treatments

目的:通过UPLC指纹图谱评价不同包装、储藏条件对羌活饮片的储藏效果,明确羌活饮片的适宜包装、储藏条件。方法:建立不同储藏时间、不同包装储藏处理羌活饮片的UPLC指纹图谱,色谱条件为采用Waters UPLC BEH C18色谱柱(2. 1 mm×50 mm,1. 7μm),流动相0. 3%磷酸水溶液(A)-乙腈(B)梯度洗脱(0~4 min,90%~47%A,;4~8 min,47%A;8~12 min,47%~20%A;12~16 min,20%A;16~18 min,20%~90%A),流速0. 2 m L·min-1,柱温35℃,检测波长246 nm,进样量1. 0μL;对指纹图谱进行相似度评价、系统聚类分析和主成分分析。结果:相似度评价显示,不同储存时间、不同包装储藏处理羌活饮片的UPLC指纹图谱谱型基本一致,相似度均> 0. 95;系统聚类显示,塑料袋包装、光照、阴凉条件下储藏的羌活饮片和初始羌活饮片聚为一类;主成分分析显示,塑料袋包装、光照、阴凉条件下储存的羌活饮片综合得分最高。结论:储存时间、包装材料、储藏温度不会引起羌活内在成分的增减,只是影响具体成分的含量;塑料袋包装、光照、阴凉条件下储存更适宜于羌活非挥发油类成分的保存。

Objective: Ultra performance liquid chromatography( UPLC) fingerprint was used to evaluate the storage effect of different packaging and storage conditions on Notopterygh Rhizoma et Radix pieces. Method:UPLC fingerprints of Notopterygh Rhizoma et Radix pieces with different storage time and packaging conditions were established under following chromatographic conditions: Waters UPLC BEH C18 column( 2. 1 mm × 50 mm,1. 7 μm),mobile phase 0. 3% phosphoric acid solution( A)-acetonitrile( B) for gradient elution( 0-4 min,90%-47% A;4-8 min,47% A,;8-12 min,47%-20% A;12-16 min,20% A;16-18 min,20%-90% A),flow rate of 0. 2 m L·min-1,column temperature at 35 ℃,detection wavelength of 246 nm,and injection volume of 1. 0 μL. The fingerprints were evaluated in terms of similarity,cluster analysis and principal component analysis.Result: The similarity evaluation showed that the UPLC fingerprint patterns of Notopterygh Rhizoma et Radix pieces with different storage time and different packaging and storage treatments were basically the same, and the similarity was above 0. 95. Systematic clustering shows that Notopterygh Rhizoma et Radix pieces packaged in plastic bags,stored under light and shade and the initial live-action pieces were clustered into one group. Principal component analysis showed that Notopterygh Rhizoma et Radix pieces packaged in plastic bags,stored under light and shade had the highest comprehensive scores. Conclusion: Storage time, packaging material and storage temperature will not cause the increase or decrease of internal components of Notopterygh Rhizoma et Radix,but only affect the content of specific components. Plastic bag packaging,light and cool storage conditions are more suitable for the preservation of non-volatile oil components of Notopterygh Rhizoma et Radix.