污泥与葡萄糖不同配比联合厌氧消化对污泥中多环芳烃去除效能及细菌群落的影响

Effect of different ratio of sludge to glucose combined with anaerobic codigestion on polycyclic aromatic hydrocarbons removal efficiency and bacterial community in sludge

为分析污泥与葡萄糖不同配比进行联合厌氧消化对污泥中多环芳烃(PAHs)去除效能及细菌群落的影响,在中温(35±1)℃条件下,以未添加葡萄糖的污泥厌氧消化为对照(CK),研究了活性污泥与葡萄糖按不同有机质含量(挥发性固体(VS)质量比)分别为1∶0.1、1∶0.3和1∶0.5对PAHs去除效能及细菌群落的影响。结果表明,葡萄糖添加量的增加并未进一步提高PAHs的降解能力。P1实验组(VS污泥∶VS葡萄糖=1∶0.1)对消化污泥中∑PAHs的去除能力最强;降解速率可达到(60.56±8.10)%;且高分子质量PAHs(≥4环)的降解速率显著高于低分子质量PAHs(2~3环)(P<0.05)。苯并(a)蒽、?、苯并(b)荧蒽和苯并(k)荧蒽的平均降解速率均大于62%;而苯并(a)芘的降解速率达到(59.60±14.05)%。此外,使用16S r RNA技术,检测消化污泥中细菌群落发现,向污泥中添加葡萄糖,可能通过促进Actinobacteria、 Bacteroidetesvadin HA17、 Spirochaetes、 Planctomycetes和norankfAnaerolineaceae菌群的生长,从而提高污泥中PAHs的去除能力。

In order to analyze the effects of the different ratio of sludge to glucose combined with anaerobic codigestion on the removal efficiency of polycyclic aromatic hydrocarbons(PAHs) and bacterial communities in sludge, at medium temperature(35±1) ℃, the effects of different organic matter contents(as volatile solids(VS)mass ratio) for sludge and glucose of 1∶0.1, 1∶0.3 and 1∶0.5 on the removal efficiency of PAHs and bacterial community were compared with anaerobic digestion of sludge without added glucose(CK). The results showed that the increase of glucose amount did not further improve the degradation ability of PAHs. Among them, P1 experimental group(VSsludgeVSglucose=1∶0.1) had the strongest removal ability of ∑PAHs in digested sludge, and degrading rate could reach(60.56±8.10)%. And the removal efficiency of high molecular weight PAHs(≥4 ring) was significantly higher than that of the low molecular weight PAHs(2~3 rings)(P<0.05). All the average degradation rates of benzo(a)pyrene, anthracene, benzo(b)fluoranthene and benzo(k)fluoranthene were higher than 62%, while the degradation rate of benzo(a)pyrene reached(59.60±14.05)%. In addition, 16 S rRNA technology was used to detect the bacterial community in the digested sludge. It was found that the addition of glucose to the sludge could enhance PAHs removal in the sludge by promoting the growth of Actinobacteria,Bacteroidetesvadin HA17, Spirochaetes, Planctomycetes and norankfAnaerolineaceae.