尿中邻苯二甲酸盐浓度与精液质量参数的相关性

Correlation between urinary phthalate concentration and semen quality parameters

目的探讨尿中邻苯二甲酸盐浓度与精液质量参数的相关性。方法选择本院就诊的112例不育症男性为不育症组,另择本院同期已生育的体检健康男性65例为对照组,采集所有受试者尿液及精液样本,采用全自动彩色精子质量检测系统检测精液质量参数,应用高效液相色谱法测定尿液中邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二丁酯(DBP)和邻苯二甲酸二异辛脂(DEHP)浓度,并采用spearman相关系数分析4种邻苯二甲酸盐浓度与精液质量参数的相关性。结果不育症组尿中DMP、DEP、DBP、DEHP及总邻苯二甲酸盐浓度均明显高于对照组(P<0.05);不育症组精子密度、精子存活率及a+b级精子、前向运动比率均明显低于对照组(P<0.05),而畸形精子比率明显高于对照组(P<0.05);尿中DMP、DEP、DBP、DEHP浓度与精子密度、精子存活率及a+b级精子、前向运动比率均呈负相关(P<0.05),与畸形精子比率呈正相关(P<0.05)。结论不育症男性尿液中邻苯二甲酸盐浓度偏高,其浓度与精液质量呈负相关,邻苯二甲酸盐蓄积可能是男性不育的重要原因之一。

Objective To explore the correlation between urinary phthalate concentration and semen quality parameters.Methods A total of 112 males with infertility in our hospital were set as infertility group.Another 65 healthy males who had given birth during the same period in our hospital were selected as control group.The semen samples obtained by urine and masturbation were collected in all the subjects.The semen quality parameters were detected by automatic color sperm quality detection system,and the concentrations of dimethyl phthalate(DMP),diethyl phthalate(DEP),dibutyl phthalate(DBP)and Di 2-Ethyl Hexyl Phthalate(DEHP)in urine were measured by high performance liquid chromatography,and the correlation between concentrations of four phthalates and semen quality parameters was analyzed by spearman correlation coefficient.Results The concentrations of DMP,DEP,DBP,DEHP and total phthalate in infertility group were significantly higher than those in control group(P<0.05).The sperm density,sperm survival rate and ratios of a+b level sperm and forward movement in infertility group were significantly lower than those in control group(P<0.05),and the ratio of abnormal sperm was significantly higher than that in control group(P<0.05).The concentrations of DMP,DEP,DBP and DEHP in urine were negatively correlated with sperm density,sperm survival rate and ratios of a+b level sperm and forward movement(P<0.05),and were positively correlated with abnormal sperm ratio(P<0.05).Conclusions The concentration of phthalate is high in urine of infertile males,and its concentration is negatively correlated with semen quality.Phthalate accumulation may be one of the important reasons for male infertility.