小鼠体内金果榄提取物古伦宾的药动学研究

Pharmacokinetics of Columbin from Tinospora Capillipes in Mice

建立高效液相色谱(HPLC)法测定小鼠血浆古伦宾浓度,并研究小鼠体内金果榄提取物古伦宾的药动学。采用灌胃的方式对小鼠给与金果榄提取物古伦宾,血浆样品用甲醇沉淀蛋白处理后经HPLC检测,色谱柱为C18柱,流动相为V(乙腈)∶V(水)=40∶60,检测波长为210 nm,流速为1.0 mL/min,柱温为35℃,进样量为20μL。古伦宾的主峰保留时间为8.66 min,峰形正态分布,分离良好,古伦宾在0.781 5~400μg/mL范围内线性关系良好,标准曲线方程为y=0.076 8x+0.293,R^2=0.999 3,最低检测限和定量限分别为0.781 5μg/mL和3.125μg/mL,日内与日间精密度均小于15%,血浆、肝脏、肾脏中的平均回收率分别为92.65%、93.88%、92.27%,各浓度水平回收率的RSD在2.53%~6.36%之间,均符合药代动力学研究要求。研究所采用的HPLC方法专属性良好,适用于古伦宾在小鼠体内的药代动力学研究。

To establish HPLC method for the determination of columbin in mice plasma and to investigate the pharmacokinetics of columbine from tinospora capillipes in mice,the columbin from tinospora capillipes were given to the mice by the method of intragastric.After precipitation by methanol,the plasma samples were detected by HPLC C18 column.Mobile phase:V(acetonitrile)∶V(water)=40∶60.Detection wavelength:210 nm.Flow rate:1.0 mL/min.Tempreture:35 ℃.Sample size:20 μL.Retention time of columbin was about 8.66 min,the peak shape was normally distributed and well separated,the liner range of columbin was from 0.781 5 μg/mL to 400 μg/mL,the standard curve equation was y=0.076 8x+0.293,R^2=0.999 3.The minimum detection limit and quantitative limit were 0.781 5 μg/mL and 3.125 μg/mL,respectively.The intra-day and inter-day precision were less than 15%,and the average recoveries in plasma,liver and kidney were 92.65%,93.88% and 92.27%,respectively.The RSD of the recoveries of each concentration was between 2.53% and 6.36%,which met the requirements of pharmacokinetic study.The HPLC method had a good specificity and was suitable for the pharmacokinetic study of columbin in mice.