玳玳花精油的成分及其抗氧化活性研究

Study on the composition and the antioxidant activity of essential oil from flowers of Citrus aurantium L. var. amara Engl

以玳玳花精油为试验材料,利用气相色谱-质谱联用技术对其进行组分分析,测定了其理化特征,采用DPPH、ABTS、羟基自由基的清除率及总抗氧化能力来评价其抗氧化活性。结果表明,玳玳花精油是一种具有玳玳花特征香气的琥珀色澄清液体,共分离出41种成分,占总成分95.80%,其中包括13种醇类化合物(59.34%),16种萜烯类化合物(22.82%),4种烷烃类化合物、3种酸、3种酯类、1种吲哚和1种酚类化合物。玳玳花精油的总抗氧化能力在4 mg/mL时超过VC;对DPPH自由基清除能力较弱,精油浓度为50 mg/mL时清除率为73.51%;对ABTS和羟基自由基清除能力较好,10mg/mL时清除率分别达到85.63%和94.87%;综上,玳玳花精油具有显著的抗氧化作用且与浓度呈正相关。

The composition of the flower essential oil fromCitrus aurantium L.var.amara Engl was analyzed by gas chromatography-mass spectrometry(GC-MS),and its physicochemical characteristics was determined.The DPPH,ABTS,hydroxyl radical scavenging rate and total antioxidant capacity were determined to evaluate the antioxidant activity of this kind of essential oil.The results showed that this essential oil was an amber clarification liquid with the characteristic aroma of Citrus aurantium L.var.amara Engl flower.Forty-one kinds of components were isolated from the essential oil,accounting for 95.80% of the total,including 13 kinds of alcohols(59.34%)and 16 kinds of terpenes(22.82%),4 kinds of alkane,three kinds of acids and ester,one kind of indole and one kind of phenol.The essential oil has a good antioxidant activity,and its total antioxidant capacity exceeded VCat high concentrations.Its scavenging ability of DPPH free radical was weak,and the scavenging rate of 50 mg/mL essential oil was 73.51%.However,Its scavenging abilities of ABTS and hydroxyl radical were better,and the scavenging ability of10 mg/mL essential oil was 85.63%and 94.87%,respectively.It was found that the antioxidant capacity of this essential oil was positively correlated with its concentration.