白藜芦醇对大鼠冻存卵巢组织自体异位移植功能影响的研究

Effects of Resveratrol on Autologous Ectopic Transplantation of Frozen Ovarian Tissues in Rats

目的:探究白藜芦醇对大鼠冻存卵巢组织自体异位移植功能的影响。方法:选取24只成熟雌性Wister大鼠,并分为空白对照组、移植组、低剂量白藜芦醇组和高剂量白藜芦醇组,每组各6只。移植组、低剂量白藜芦醇组和高剂量白藜芦醇组采用手术摘取卵巢,使用玻璃化冷冻保存2周。低剂量白藜芦醇组和高剂量白藜芦醇组卵巢组织在白藜芦醇溶液中平衡,且在大鼠冻存卵巢组织自体异位移植前、后分别腹腔注射5、10μmol/L白藜芦醇处理,移植组注射等量生理盐水。使用HE染色后光镜下观察卵泡情况,并记录各组大鼠异常卵泡数目百分比。记录各组大鼠月经恢复情况,检测各组大鼠血清内氧化应激相关物质超氧化物歧化酶(SOD)、活性氧基团(ROS)含量水平。分别于第4、8周检测各组大鼠血清激素E2及P的分泌情况。结果:相比空白对照组,移植组异常卵泡数目百分比、ROS含量水平均显著升高,SOD含量及第4、8周大鼠E2及P含量均显著降低(P<0.05);相比移植组,低剂量白藜芦醇组和高剂量白藜芦醇组异常卵泡数目百分比、月经恢复时间、ROS含量水平均显著降低,第4、8周E2、P和SOD含量均显著升高(P<0.05)。结论:白藜芦醇对大鼠冻存卵巢组织自体异位移植功能恢复有促进作用,同时可以降低大鼠氧化应激反应保护大鼠卵巢。

Objective:To investigate the effects of Resveratrol on autologous ectopic transplantation of frozen ovarian tissues in rats.Method:A total of 24 mature female Wister rats were enrolled and divided into blank control group,transplantation group,low-dose Resveratrol group and high-dose Resveratrol group,6 cases in each group.The transplantation group,low-dose Resveratrol group and high-dose Resveratrol group were given surgery to obtain ovaries.And they were preserved by vitrification for 2 weeks.Before and after autologous ectopic transplantation of frozen ovarian tissues in rats,low-dose Resveratrol group and high-dose Resveratrol group were intraperitoneally injected with 5 and 10μmol/L Resveratrol.The transplantation group was injected with same amount of Normal Saline.After HE staining,follicles were observed under light microscope.The percentage of abnormal follicles number in each group was recorded,the recovery of menstruation in each group was recorded,the levels of oxidative stress related substances such as superoxide dismutase(SOD)and reactive oxygen species(ROS)in serum of each group were detected.On the 4th week and 8th week,secretion of serum hormones E2 and P in each group were detected.Result:Compared with the blank control group,percentage of abnormal follicles number and ROS level were significantly increased in transplantation group.The levels of SOD E2 and P on 4th week and 8th week were significantly decreased(P<0.05).Compared with the transplantation group,percentage of abnormal follicles number,recovery time of menstruation and ROS level were significantly decreased in low-dose Resveratrol group and highdose Resveratrol group,the levels of E2 and P on 4th week and 8th week and SOD level were significantly increased(P<0.05).Conclusion:Resveratrol can promote function recovery of autologous ectopic transplantation of frozen ovarian tissue in rats,and reduce oxidative stress response to protect rat ovaries.