镇巴县仿野生栽培大黄的基地优选及产品质量评价

Regional optimization and product quality evaluation of Rheum officinale Baill.imitation wild planting in Zhenba Country

目的同时测定镇巴县不同种植基地大黄中多个化学成分的含量,与市售大黄作比较,进行质量评价,确定大黄优质产区。方法采用HPLC法。岛津ODS-HL C 18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-1 mL·L^-1磷酸溶液,梯度洗脱;检测波长为254 nm;流速为1.0 mL·min^-1;柱温为30℃。结果建立同时测定大黄中11个化学成分含量的HPLC法,对镇巴县不同种植基地大黄的质量进行比较分析。结果显示,各基地大黄不同成分含量差异较大,与市售大黄相比,鞣质类成分儿茶素的含量较高,在选择种植基地时应避开九阵中坝村。结论建立的方法简便、准确、可靠,可用于大黄的质量控制。

Objective To analyze the content of several chemical components in Rheum officinale Baill.in different planting bases in Zhenba Country,to evaluate the quality of R.officinale Baill.,to compare with the rhubarb on the market,and to determine the quality of production areas in Zhenba Country.Methods HPLC was used with Shimadzu ODS-HL C 18(250 mm×4.6 mm,5μm)column.The mobile phase was acetonitrile-1 mL·L-1 H 3 PO 4 at a flow rate of 1.0 mL·min-1 in a gradient elution mode.The column temperature was 30℃.The detection wavelength was 254 nm.Results A simultaneous HPLC determination method for 11 chemical components in R.officinale Baill.was established.The quality of R.officinale Baill.in different planting bases in Zhenba Country was compared and analyzed.The results showed that the contents of different components of R.officinale Baill.in different bases were significantly different.The content of catechin planted in Zhenba Country was higher than that in commercial R.officinale Baill..Zhongba village should not be selected as the R.officinale Baill.planting base.Conclusion The established analytical method is simple,accurate and reliable.The method could be used to control the quality of R.officinale Baill..