胃癌患者血清miR-378和miR-21表达变化及其诊断效能研究

Changes of Serum miR-378 and miR-21 Expression in Patients with Gastric Cancer and Their Diagnostic Efficiency

目的:研究胃癌患者血清微小核糖核酸-378(miR-378)和微小核糖核酸-21(miR-21)表达变化及其诊断效能。方法:选择2015年3月-2017年12月本院收治的74例胃癌患者为胃癌组,同期收集62例胃良性病变患者为胃良性病变组,另选择本院门诊健康体检者69例为对照组。比较各组血清miR-378和miR-21表达情况,分析胃癌患者血清miR-378和miR-21和临床病理特征的关系,不同预后组血清miR-378和miR-21表达情况,并分析以上指标对胃癌的诊断效能。结果:三组血清miR-378和miR-21比较,差异均有统计学意义(P<0.05);对照组血清miR-378均高于胃良性病变组和胃癌组,且胃良性病变组高于胃癌组(P<0.05);对照组血清miR-21均低于胃良性病变组和胃癌组,且胃良性病变组低于胃癌组(P<0.05)。胃癌患者血清miR-378和miR-21的表达与年龄、性别均无关(P>0.05),而与肿瘤直径、组织分化程度、TNM分期、肿瘤浸润深度、淋巴结转移均有关(P<0.05)。预后不良组血清miR-378水平低于预后良好组,miR-21水平高于预后良好组,差异均有统计学意义(P<0.05)。制作miR-378、miR-21、miR-378+miR-21对胃癌诊断的ROC曲线,其曲线下面积分别为0.802、0.774、0.882,灵敏度分别为0.812、0.779、0.841,特异度分别为0.703、0.770、0.824。结论:血清miR-378在胃癌患者中表达下降,miR-21表达明显上调,miR-378联合miR-21在胃癌诊断中有较高的诊断效能,可作为胃癌诊断的参考依据。

Objective:To study the expression of microRNA-378 (miR-378) and microRNA-21 (miR-21) in serum of patients with gastric cancer and their diagnostic efficacy.Method:A total of 74 patients with gastric cancer admitted to our hospital from March 2015 to December 2017 were selected as gastric cancer group,at the same time,62 patients with gastric benign lesions were collected as gastric benign lesions group,in addition,69 outpatients were selected as the control group.The expression of miR-378 and miR-21 were compared,the relationship between serum miR-378 and miR-21 and clinicopathological characteristics of gastric cancer patients,the expressions of serum miR-378 and miR-21 in different prognosis groups were analyzed,and the diagnostic efficacy of the above indicators for gastric cancer was analyzed.Result:The serum miR-378 and miR-21 of the three groups were compared,the difference was statistically significant (P<0.05).The serum miR-378 of the control group were higher than those of the gastric benign lesion group and the gastric cancer group,the benign lesion group was higher than the gastric cancer group (P<0.05).The serum miR-21 of the control group were lower than those of the gastric benign lesion group and the gastric cancer group,and the benign lesion group was lower than the gastric cancer group (P<0.05).The expression levels of serum miR-378 and miR-21 in gastric cancer patients were not correlated with age and gender (P>0.05),but they were correlated with tumor diameter,tissue differentiation,TNM stage,tumor invasion depth and lymph node metastasis (P<0.05).The level of miR-378 in the poor prognosis group was lower than that in the good prognosis group,and the level of miR-21 was higher than that in the good prognosis group (P<0.05).The ROC curves of miR-378,miR-21 and miR-378 + miR-21 in the diagnosis of gastric cancer were made,the area under the curve were 0.802,0.774 and 0.882,the sensitivity were 0.812,0.779 and 0.841,and the specificity were 0.703,0.770 and 0.824.Conclusion:The expression of miR-378 in serum decreased and miR-21 was up-regulated in patients with gastric cancer.The combination of miR-378 and miR-21 has a high diagnostic efficiency in the diagnosis of gastric cancer,which can be used as a reference for the diagnosis of gastric cancer.