高效液相色谱法测定普洱熟茶中Puerins 401系列化合物的含量

Determination of Puerins 401 Series Compounds in Pu-erh Tea by HPLC

文章建立了测定普洱熟茶中Puerins 401系列化合物含量的高效液相色谱分析方法。普洱熟茶中Puerins 401系列化合物用水提取,经二氯乙烷萃取,HP20自装柱富集净化,净化液经Waters Atlantis dC18色谱柱分离,以2 mmol/L乙酸铵水溶液(含1‰甲酸)和乙腈为流动相进行梯度洗脱,检测波长280 nm,流速1 mL/min,外标法定量。结果表明,在2.0~500.0 mg/L范围内,Puerins 401系列化合物线性关系良好,相关系数0.999。Puerins 401系列化合物最低检出限为3.6 mg/kg,最低定量限为11.9 mg/kg,加标回收率75.0%~99.5%,相对标准偏差均小于5%。该方法灵敏度高,准确性好,适用于普洱熟茶中Puerins 401系列化合物的测定。

In this paper,a High Performance Liquid Chromatography method for the determination of Puerins 401 series compounds in Pu-erh tea was established.Puerins 401 in Pu-erh tea was extracted with water,then extracted with dichloroethane,and purified by home-made HP20 clean-up column.The purified solution was separated by Waters Atlantis dC18 column with 2 mmol/L ammonium acetate aqueous(1‰formic acid)and acetonitrile as mobile phase for gradient elution.The detection wavelength was 280nm,the flow rate is 1 mL/min,and the external standard method was used for quantification.The results showed that the linear relationship of Puerins 401 series compounds in the range of 2.0~500.0 mg/L was good,and the correlation coefficient was 0.999.The minimum detection limit of Puerins 401 series compounds was 3.6 mg/kg,the minimum limit of quantification was 11.9 mg/kg,the recoveries were 75.0%~99.5%,and the relative standard deviations were less than 5%.The method is sensitive and accurate,and is suitable for the determination of Puerins 401 series compounds in Pu-erh Tea.