口服益生菌改善铝诱发的小鼠海马炎症

Oral Probiotics Improves Aluminum-induced Hippocampal Inflammation in Mice

[目的]探讨口服益生菌能否改善铝暴露诱发的小鼠海马炎症.[方法]通过给予小鼠口服氯化铝,建立海马炎症模型,将24只8周龄雄性小鼠C57BL/6随机分成对照(CON)组、氯化铝处理(Al)组、益生菌处理(PO)组和联合处理(Al+PO)组,每组各6只,分别持续给予口服氯化铝和(或)益生菌6周.海马神经炎症的评估,采用免疫荧光染色法检测小鼠海马组织中激活的小胶质细胞(Iba-1+/CD68+)数量以及脑源性神经营养因子(BDNF)的表达量,酶联免疫吸附测定法检测血清和海马组织中白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的含量.[结果]免疫荧光染色法研究结果显示:与CON组相比,Al组海马组织中Iba-1+/CD68+细胞数量显著增加(P<0.01),而BDNF表达量显著减少(P<0.01);与Al组相比,Al+PO组海马组织中Iba-1+/CD68+细胞数量显著减少(P<0.01),而BDNF表达量增加(P<0.01).酶联免疫吸附测定(ELISA)研究结果显示:与CON组相比,Al组血清和海马组织中的IL-1β和TNF-α含量均有显著增加(P<0.01);与Al组相比,Al+PO组血清IL-1β含量显著减少(P<0.01),海马组织中的IL-1β和TNF-α含量均显著减少(P<0.01).[结论]口服益生菌可改善铝暴露诱发的小鼠海马炎症.

【Objective】To investigate whether oral administration of probiotics could improve the aluminum-induced hippocampal inflammation in mice.【Methods】Twenty-four 8-week-old male C57BL/6 mice were randomly divided into 4 groups,6 in each.The mice in control(CON)group,AlCl3-treated(Al)group,probiotics-treated(PO)group and treatment-combined(Al+PO)group were treated with sterile water,oral AlCl3,probiotics in sterile water and a combination of oral AlCl3 and probiotics in sterile water,respectively.After six weeks of treatment,immunofluorescence staining was used to test the numbers of activated microglia(Iba-1+/CD68+cells)and the expression level of brain-derived neurotrophic factor(BDNF)in hippocampus;enzyme linked immunosorbent assay(ELISA)was employed to determine the levels of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in serum and hippocampus.【Results】The morphology revealed that compared with those in CON group,in Al group,the numbers of Iba-1+/CD68+cells increased significantly(P<0.01)and the BDNF level decreased significantly(P<0.01).Compared those in Al group,in Al+PO group,the numbers of Iba-1+/CD68+cells were significantly lower(P<0.01)and the BDNF level significantly higher(P<0.01).ELISA results showed that compared with those in CON group,in Al group,the levels of IL-1βand TNF-αin serum and hippocampus had a significant rise(P<0.01).Compared those in Al group,in Al+PO group,the levels of IL-1βin serum and hippocampus and TNF-αin hippocampus had a significant reduction(P<0.01).【Conclusions】Oral probiotics improves the aluminum-induced hippocampal inflammation in mice.