食管鳞癌患者唾液菌群的研究

Analysis of Saliva Microbiome in Patients with Esophageal Squamous Cell Carcinoma

[目的]分析食管鳞癌患者(ESCC组)与健康对照组(HC组)唾液菌群多样性,筛选出食管鳞癌患者唾液中特异性细菌.[方法]收集50例食管鳞癌患者和40例健康对照者的唾液标本,提取总DNA,用PCR扩增16S rDNA V4区,进行高通量测序,将测序数据进行OUT聚类,物种注释及多样性分析.[结果]多样性分析表明ESCC组和HC组整体唾液菌群的多样性及组成方面存在差异(但P>0.05),物种分析表明ESCC组以奈瑟菌属(Neisseria)、梭杆菌属(Fusobacterium)、嗜血杆菌属(Haemophilus)、普式菌属(Prevotella)、卟啉单胞菌属(Porphy?romonas)为主,健康对照组以奈瑟菌属(Neisseria)、嗜血杆菌属(Haemophilus)、普式菌属(Prevotella)、梭杆菌属(Fusobacterium)、韦荣氏球菌属(Veillonella)为主,LEfSe分析表明ESCC组卟啉菌属表达升高,且具有统计学差异(P<0.05).[结论]食管癌患者唾液有其特征性的菌群组成,与健康对照组相比,食管鳞癌患者唾液中卟啉菌属表达明显升高,是特征性表达的细菌.

【Objective】To analyze the difference of salivary microbiome between patients with esophageal squamous cell carcinoma(ESCC group)and healthy controls(HC group),and to screen out the target bacteria in saliva of patients with esophageal squamous cell carcinoma.【Methods】Salivary samples were collected from 50 patients with esophageal squamous cell carcinoma and 40 healthy controls.Total DNA was extracted,16S rDNA V4 region was amplified by PCR,high-throughput sequencing was performed,and sequencing data was subjected to OUT clustering,species annotation and diversity analysis.【Results】Diversity analysis showed difference in the diversity of the salivary microbiome between the ESCC group and the HC group,but P>0.05.Species analysis showed that the salivary microbiome in the ESCC group was dominated by Neisseria,Fusobacterium,Haemophilus,Prevotella and Porphyromonas,while Neisseria,Haemophilus,Prevotella,Fusobacterium and Veillonella were the top microbiota in the HC group.LEfse analysis showed that the expression of Porphyromonas in the ESCC group was elevated and the result had statistical significance.【Conclusion】Saliva in the patients with esophageal squamous cell carcinoma has characteristic microbiome composition.Compared with the healthy control group,the expression of Porphyromonas in saliva of patients with esophageal squamous cell carcinoma is significantly increased,and the result is statistically significant(P<0.05).