玉米种子内部拟轮枝镰孢菌抗性的QTL定位

QTL Mapping of Seed Internal Fusarium verticillioides Resistance in Maize

为探究玉米种子内部对拟轮枝镰孢菌的抗性,利用由抗病自交系BT-1与感病自交系N6构建的RIL群体为材料,进行4个环境的联合QTL分析.结果表明,6个控制种子内部对拟轮枝镰孢菌抗性的QTL分布在第1、3、4、9、10染色体上,表型贡献率介于1.32%~7.62%.其中,表型贡献率最高的QTL qSIR3-1位于第3染色体,解释7.62%的表型贡献率,抗病效应来自于抗病亲本BT-1.6个QTL中的1个与已知种子外部抗性QTL一致,表明玉米种子内部对拟轮枝镰孢菌的抗性是独立的性状.

In order to explore the seed internal resistance of maize to Fusarium verticillioides,RIL populations constructed from disease resistant inbred line BT-1 and susceptible inbred line N6 were used and phenotypes were surveyed in four environments to identify resistance QTLs.The results showed that 6 QTLs were located on chromosomes 1,3,4,9 and 10,with phenotypic contribution rate from 1.32%to 7.62%.The resistance QTL qSIR3-1 derived from BT-1 with the greatest contribution rate was located on chromosome 3,which could explain 7.62%of phenotypic variance.One of six identified QTLs was consistent with seed external resistance,showing that the internal resistance of seeds to F.verticillioides differed from other tissue resistance with some conservative mechanism.