一氧化氮供体SNP对不同苜蓿根瘤菌菌株生长的影响

Effect of nitric oxide donor SNP on the growth of different alfalfa rhizobium strains

为探寻提高苜蓿-根瘤菌共生固氮能力的途径,以苜蓿中华根瘤菌为材料,用SNP为外源NO供体,研究不同浓度外源NO对根瘤菌生长的影响及不同根瘤菌菌株对外源NO的敏感性差异,以期筛选出适宜根瘤菌生长的外源NO浓度和可与NO配施的根瘤菌菌株。结果表明:SNP浓度为0.08 mmol/L时根瘤菌数量显著高于其他处理,其D_(600 nm)值为0.9680;外源NO对于根瘤菌TA34的生长有明显的促进作用,D_(600 nm)值较CK提高了46.01%,而对WL68则具有较为显著的抑制性,D_(600 nm)值较CK降低了71.63%;不同菌种对于NO的敏感度不同,根瘤菌WL68对外源NO最敏感,而根瘤菌GL28对外源NO不敏感。

In order to explore approaches to improve the nitrogen fixation ability of alfalfa rhizobia,the effects of different concentrations of exogenous NO on the growth of rhizobia and the sensitivity of different rhizobia to exogenous NO were studied by using SNP as the donor of exogenous NO to screen out the suitable concentration of exogenous NO for the growth of rhizobia and the rhizobia strains that can be combined with NO.The results showed that when SNP concentration was 0.08 mmol/L,the number of rhizobia was significantly higher than that of other treatments,and its OD value was 0.9680.Exogenous NO promoted the growth of TA34 significantly,and the OD value increased 46.01%compared with CK,but it inhibited WL68 significantly,and the OD value decreased 71.63%compared with CK.Different strains showed different sensitivities to NO,and WL68 was the most sensitive to exogenous NO,while GL28 was not sensitive.