水稻品种‘扬稻二号’与‘扬稻六号’的抗旱性差异分析

The Variance Analysis of Drought-Resistant between Rice Cultivar’Yangdao-2’(YD2)and’Yangdao-6’(YD6)

为分析‘扬稻二号’与‘扬稻六号’的抗旱性差异,以抗旱性不同的两个籼稻品种(‘扬稻二号’与‘扬稻六号’)为研究对象,检测了叶片的保水力及表皮渗透度,并使用扫描电镜观察了叶片表面的超微结构,同时检测了耐逆相关基因在干旱处理前后的表达动态。结果表明:在离体情况下‘扬稻六号’叶片的失水率和表皮渗透度都低于‘扬稻二号’;‘扬稻六号’叶片表面的气孔长度小于‘扬稻二号’,但单位面积内的硅质乳突、气孔及气孔周沿乳突数均高于‘扬稻二号’;一部分干旱相关基因在品种间共同诱导表达,如OsPP2C和OsDHN,而另外一部分基因在品种间差异表达,如OsSTPK-1、OsNAC6和OsZF1。这些结果为进一步阐明‘扬稻二号’与‘扬稻六号’的抗旱性差异提供了新的依据。

To analyze drought tolerance rice cultivar Yangdao-2(YD2)and Yangdao-6(YD6),our previous study found that YD6 showed better drought tolerance at the seedling stage than YD2 did.Here we further checked the ultrastructure of leaf surface and expression of stress-related genes between two rice varieties.Consistent with the phenotype,the detached leaves of YD6 showed lower water loss rate and Chlorophyll efflux rate than YD2 did.Using the electric scanning microscope,we found that the leaf surface of YD6 exhibited smaller but more stomata as compared with YD2.In addition,the number of tubercles in YD6 was greater than YD2 both in leaf surface and around the stomata.Using the semi-quantitative RT-PCR,we found that some stress-related genes with multiple functions showed higher expression or stronger induction by drought stress in YD6 than that in YD2.