山药蛋白肽对免疫能力低下小鼠的免疫调节作用

Immunomodulatory effects of the iron yam peptides on mice with immunocompromised

该文研究山药蛋白肽对免疫能力低下小鼠的免疫调节作用。试验采用环磷酰胺(cyclophospamide,CY)皮下注射构建免疫功能低下小鼠模型,通过口腔灌胃低、中、高不同剂量的山药蛋白肽,并与空白对照组、阴性对照组相比较,通过免疫器官、免疫细胞、免疫活性物质三方面探讨山药蛋白肽对免疫能力低下小鼠的免疫调控作用和作用机制。结果表明,山药蛋白肽含有极其丰富的疏水性氨基酸和碱性氨基酸;在小鼠免疫器官方面,CY构建免疫能力低下小鼠模型的免疫器官指数指标显著降低,脾脏发生明显的病理学变化,山药蛋白肽能促进免疫器官指数的提高,并且对脾脏病理学变化有显著改善作用;在小鼠免疫细胞方面,CY阴性对照组的乳酸脱氧酶(lactic dehydrogenase,LDH)和酸性磷酸酶(acid phosphatase,ACPase)活性、淋巴细胞增殖能力显著下降,山药蛋白肽能显著提高LDH、ACPase酶活性和淋巴细胞增殖能力;在小鼠免疫活性物质方面,CY阴性对照组的细胞因子(IL-1α、IL-6、IFN-γ)和免疫球蛋白(IgG、IgM)水平均显著低于正常组,山药蛋白肽能显著提升IL-1α、IL-6、IFN-γ水平和IgG、IgM水平,这表明山药蛋白肽能通过激活和保护免疫系统中的免疫器官、免疫细胞和免疫活性物质发挥免疫调节作用,进而增强机体的免疫防御能力。

The immunomodulatory effect of the iron yam peptides(IYPs)on immunocompromised mice and its mechanism of action were studied.In this study,cyclophosphamide(CY)was used to establish immunocompromised mouse model.The iron yam peptides were given orally by gavage and cyclophosphamide was injected subcutaneously every other day.The immunomodulatory effects of iron yam peptides on CY-immunized mice were investigated from the body's immune system.The results showed that IYPs contained abundant hydrophobic and essential amino acids.In the immune organs of mice,the immune organ index of CY-immunized mouse model was significantly decreased and the pathological changes of spleen occurred.The phaseolus vulgaris peptides also could promote the index of immune organs and pathology of spleen.The improvement was significant,which indicated that iron yam peptides could promote the development of immune organs in mice with immunocompromised.In mouse immune cells,LDH and ACPase activities,lymphocyte proliferation was significantly decreased in CY-immunized control group.The iron yam peptides significantly increased LDH,ACPase activity and lymphocyte proliferation.In the mouse immunosuppressive substances,the cytokines(IL-1α,IL-6,IFN-γ)and immunoglobulin(IgG,IgM)levels in the CY-immunized group were significantly lower than the normal group.The iron yam peptides significantly increased IL-1α,IL-6,IFN-γlevels and IgG,IgM levels.The iron yam peptides can exert immunomodulatory effects by activating and protecting immune organs,immune cells and immune active substances,thereby enhancing immune ability.