Vero细胞最佳培养体系筛选

The Screening of Optimal Culture System for Vero Cells

为了筛选出适合Vero细胞生长的最佳条件,本研究分别对Vero细胞的培养液、传代比例和接种浓度进行了优化.首先将Vero细胞在不同组合培养液中进行连续3代传代培养,筛选出适宜Vero细胞连续稳定传代的最佳培养液组成.然后分别在不同的传代比例和接种浓度下进行连续3代培养,筛选出适宜Vero细胞连续稳定传代的最佳传代比例和接种浓度.最终结果显示,Vero细胞在1.296%MEM溶液与特级胎牛血清的组合优于其他组合,可满足Vero细胞连续稳定传代.并且在该培养基的培养下,1∶6的传代比例为最适宜Vero细胞的传代比例,3×10^4/cm^2的接种密度为最适宜Vero细胞的接种密度.本研究最终完成了Vero细胞最佳培养体系条件筛选,并证实该条件满足Vero细胞连续稳定传代.

In order to screen the optimal culture system for Vero cell culture, this study has optimized the culture medium, passage ratio and density for three consecutive passage respectively. Above all, Vero cells were cultured in different kinds of medium for three passages to select the best medium. Subsequently, different subculture density and proportion of Vero cells were cultured in the optimal medium for three passage, and the optimized passage ratio win out. In general, 1.296% MEM medium supplied with superfine fetal bovine serum is better than the other combination of medium and it can sustain the successive stable culture passaging. In the condition of this medium, the best proportion of cell passaging is 1∶6 and the optimal cell density is 3×10^4/cm^2. In this study,we screened the conditions of the best Vero cell culture system and confirmed that the conditions meet the continuous and stable passage of Vero cells.