摘要
高胆固醇血症为心脑血管疾病的重要诱因,使用具有降胆固醇活性的乳酸菌调控血清胆固醇水平具有重要意义。研究从健康人群粪便中分离获得1株动物双歧杆菌和5株屎肠球菌,考察了这些菌株的胆固醇脱除能力、胆盐耐受能力、模拟胃肠道条件耐受能力等特性。结果表明,屎肠球菌MCLF03和MCLF04对胆固醇的脱除能力显著高于其他菌株,脱除率分别为(38.9±3.77)%和(61.0±4.75)%。屎肠球菌MCLF04在0.5%胆盐溶液、人工胃液、人工肠液中的耐受性均高于屎肠球菌MCLF03。屎肠球菌MCLF04具有潜在的降胆固醇特性,可用于降胆固醇功能性食品的开发研究。
Hypercholesterol is an important inducement of cardiovascular and cerebrovascular diseases.It is significance to use lactic acid bacteria with cholesterol lowering activity to regulate serum cholesterol level.In this study,one strain of Bifidobacillus animalis and five strains of Enterococcus faecium were isolated from health human feces.The cholesterol removal ability,bile salt tolerance and gastric acid tolerance of these strains were investigated.The results showed that the cholesterol removal ability of Enterococcus faecium MCLF03 and MCLF04 was significantly higher than those of other strains,with the removal rates (38.9±3.77)% and (61.0±4.75)%,respectively.The tolerance of E.faecium MCLF04 to 0.5% bile salt solution,artificial gastric juice and artificial intestinal fluid was higher than those of E.faecium MCLF03.E.faecium MCLF04 has cholesterol-lowering potential and can be applied in functional food development.
作者
马长路
田玲
罗红霞
刘小飞
李凌燕
杨新建
王立晖
焦扬
MA Changlu;TIAN Ling;LUO Hongxia;LIU Xiaofei;LI Lingyan;YANG Xinjian;WANG Lihui;JIAO Yang(Department of Food and Bio-engineering,Beijing Vocational College of Agriculture,Beijing 102442,China;Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Agriculture and Biotechnology,Hexi University,Zhangye 734000,China;Tianjin Modern Vocational Technology College,Tianjin 102206,China)
出处
《食品科技》
CAS
北大核心
2020年第2期23-28,共6页
Food Science and Technology
基金
北京市教委科技计划项目(KM201812448003)
国家重点研发计划专项(2017YFC1600903)
国家自然科学基金面上项目(31871833、31371808)
教育部食品营养与安全应用技术协同创新中心项目(XM-16-H23)
万人计划教学名师特支项目(2018年11月)。
关键词
粪便
肠球菌
胆固醇降低
feces
Enterococcus
cholesterol-lowering
