新型冠状病毒核酸提取与扩增试剂匹配的检测结果分析

Analysis of detection results of matching nucleic acid extraction and amplification reagents for 2019-nCoV nucleic acid

目的比较分析新型冠状病毒核酸提取试剂与扩增试剂匹配与否的结果。方法收集我院23例疑似和确诊患者送检的咽拭子样本,采用A、B两家公司的提取试剂分别提取RNA,匹配扩增试剂和交叉扩增试剂进行检测,根据检测结果比较分析试剂匹配与否的检测结果差异。结果提取试剂与匹配的扩增试剂检出率明显高于试剂交叉检测的检出率(匹配组检出率在30%以上,交叉组检出率在20%以下)。结论新型冠状病毒核酸提取试剂与扩增试剂匹配使用检测效果更好。

Objective To compare and analyze whether nucleic acid extraction reagent and amplification reagent of novel coronavirus(2019-nCoV)were matched or not.Methods Samples of pharynx swabs from 23 suspected and confirmed patients in our hospital were collected,and RNA was extracted with the extraction reagent of two companies A and B and the matching amplification reagent and the cross-amplification reagent were used for detection.The test results of reagents matching or not were compared and analyzed.Results The positive rate of the extracted reagent and the matched amplified reagent was significantly higher than that of the crossed reagents.The percentage of positive test was more than 30%in the matched group and less than 20%in the cross group.Conclusion The detection effect of 2019-nCoV nucleic acid is better when nucleic acid extraction reagent and amplification reagent are matched.