红细胞分布宽度对气虚质大鼠辅助诊断的评价

Evaluation of red cell distribution width in the assistant diagnosis of Qi deficiency in rats

目的探讨红细胞分布宽度(RDW)对气虚质的辅助诊断价值。方法将60只SD大鼠随机数字表法分为脾气虚质组(A组)和对照组(B组)各30只。A组大鼠采用限制饮食结合干扰睡眠的方法进行造模,造模成功后比较各组RDW、血红蛋白(Hb)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)差异,建立受试者工作特征曲线(ROC曲线)分析检测指标单独和联合检测时对气虚质的诊断价值。结果A组TNF-α、IL-1β、RDW明显高于B组,差异有统计学意义(P<0.05),但两组Hb比较差异无统计学意义(P>0.05)。ROC曲线分析显示,单独检测时,RDW对气虚质诊断效能明显高于IL-1β及TNF-α(P<0.05)。RDW单独检测和与IL-1β、TNF-α两项或三项联合检测时,诊断效能差异无统计学意义(P>0.05)。当RDW=17.6%为最佳界值点时,敏感性为80%,特异性为80%,阳性预测值为80%,阴性预测值为80%。结论RDW与气虚质有直接关系,在识别气虚质时是一个有价值的早期预警指标。

Objective To evaluate the assistant diagnostic value of red cell distribution width(RDW)in Qi deficiency.Methods Sixty SD rats were randomly divided into group A(spleen Qi deficiency group)and group B(control group),30 in each group.In the group A,rats were modeled by diet restriction combined with sleep disturbance.After successful modeling,we compared the difference of RDW,hemoglobin(Hb),tumor necrosis factor-α(TNF-α)and interleukin-1beta(IL-1β)in each group.Receiver operating characteristic curve(ROC curve)was constructed to analyze the diagnostic values of single and combined detection of these indexes for spleen Qi deficiency.Results The levels of RDW,IL-1βand TNF-αin the group A were significantly higher than those in the group B(P<0.05),but there was no significant difference in Hb between the two groups(P>0.05).According to the ROC curve analysis,the diagnostic performance of RDW(AUC=0.872)was significantly higher than that of IL-1β(AUC=0.667)and TNF-α(AUC=0.670)for Qi deficiency by using single detection(P<0.05).There was no significant difference in diagnostic performance between RDW alone and two or three combined tests with IL-1βand TNF-α(P>0.05).When 17.6%RDW was taken as the optimal cut-off point,the sensitivity was 80%,the specificity was 80%,the positive predictive value was 80%and the negative predictive value was 80%,respectively.Conclusion RDW is directly associated with Qi deficiency,which is a valuable early warning index in identifying Qi deficiency.