番茄褪绿病毒侵染黄瓜的首次报道

Cucumber(Cucumis sativus)is a new host for Tomato chlorosis virus

番茄褪绿病毒Tomato chlorosis virus(ToCV)是严重危害世界经济作物的一种病毒,寄主范围广泛。田间调查发现黄瓜Cucumis sativus表现出叶片黄化、脉间褪绿的疑似番茄褪绿病毒感病症状,同时叶片背面聚集了大量烟粉虱。采用RT-PCR方法对样品叶片和烟粉虱进行检测,ToCV感染率为65%,且发病叶片上烟粉虱携带ToCV。为进一步确定黄瓜是否为番茄褪绿病毒的新寄主,室内利用农杆菌侵染性克隆接种健康黄瓜,结果显示:接种30 d的黄瓜新生叶片出现褪绿症状。采用ToCV HSP70基因的引物对田间黄瓜叶片、烟粉虱和室内黄瓜新生叶片进行RT-PCR,扩增出约450 bp的条带,在NCBI上BLAST显示与KC887999.1的同源性最高,为99%。这些数据表明黄瓜是番茄褪绿病毒的寄主。这是ToCV感染黄瓜的首次报道。

Tomato chlorosis virus(ToCV)causes severe damage to crops worldwide,and it infects a wide range of plant hosts.In a field investigation,we found leaf etiolation,interveinal chlorosis of suspected symptoms of tomato chlorosis virus disease in cucumber(Cucumis sativus);meanwhile a large number of Bemisia tabaci gathered on the back of cucumber leaves.The leaves and B.tabaci were tested by RT-PCR.The results showed that the ToCV infection rate was 65%,and B.tabaci on the infected leaves carried ToCV.In order to further determine whether cucumber is the new host of tomato chlorosis virus,healthy cucumbers were inoculated with agrobacterium-infected clones indoors.The results showed that chlorosis appeared in new leaves of cucumbers 30 days after inoculation.The primers of ToCV HSP70 gene were used to perform RT-PCR on cucumber leaves,whiteflies and new leaves of indoor cucumber,and a band of 450 bp was amplified.BLAST on NCBI showed the highest homology(99%)with KC887999.1.These data indicate that cucumber is the host of tomato chlorosis virus.This is the first report of ToCV infection in cucumbers.